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Evolution of an X-Linked miRNA Family Predominantly Expressed in Mammalian Male Germ Cells

文献类型：期刊论文

作者Zhang, Fengjuan1,2,3; Zhang, Ying1,2,3; Lv, Xiaolong1,2,3; Xu, Beiying1,2,3; Zhang, Hongdao1,2,3; Wu, Ligang1,2,3; Yan, Jun4; Li, Haipeng5; ,
刊名MOLECULAR BIOLOGY AND EVOLUTION
出版日期2019
卷号36期号:4页码:663-678
关键词X-linked miRNAs THER1 spermatogenesis evolution meiosis transposable element MSCI
ISSN号0737-4038
DOI10.1093/molbev/msz001
文献子类Article
英文摘要MicroRNAs (miRNAs) are important posttranscriptional regulators of gene expression. However, comprehensive expression profiles of miRNAs during mammalian spermatogenesis are lacking. Herein, we sequenced small RNAs in highly purified mouse spermatogenic cells at different stages. We found that a family of X-linked miRNAs named spermatogenesis-related miRNAs (spermiRs) is predominantly expressed in the early meiotic phases and has a conserved testis-specific high expression pattern in different mammals. We identified one spermiR homolog in opossum; this homolog might originate from THER1, a retrotransposon that is active in marsupials but extinct in current placental mammals. SpermiRs have expanded rapidly with mammalian evolution and are diverged into two clades, spermiR-L and spermiR-R, which are likely to have been generated at least in part by tandem duplication mediated by flanking retrotransposable elements. Notably, despite having undergone highly frequent lineage-specific duplication events, the sequences encoding all spermiR family members are strictly located between two protein-coding genes, Slitrk2 and Fmr1. Moreover, spermiR-Ls and spermiR-Rs have evolved different expression patterns during spermatogenesis in different mammals. Intriguingly, the seed sequences of spermiRs, which are critical for the recognition of target genes, are highly divergent within and among mammals, whereas spermiR target genes largely overlap. When miR-741, themost highly expressed spermiR, is knocked out in cultured mouse spermatogonial stem cells (SSCs), another spermiR, miR-465a-5p, is dramatically upregulated and becomes the most abundant miRNA. Notably, miR-741(-/-) SSCs grow normally, and the genome-wide expression levels of mRNAs remain unchanged. All these observations indicate functional compensation between spermiR family members and strong coevolution between spermiRs and their targets.
学科主题Biochemistry & Molecular Biology ; Evolutionary Biology ; Genetics & Heredity
WOS关键词SMALL RNAS ; MOTILE CILIOGENESIS ; MICRORNA CLUSTER ; RAPID EVOLUTION ; SEX-CHROMOSOMES ; RECENT ORIGIN ; ALU REPEATS ; MOUSE ; IDENTIFICATION ; SEQUENCE
语种英语
WOS记录号WOS:000473583700003
出版者OXFORD UNIV PRESS
版本出版稿
源URL[http://202.127.25.144/handle/331004/1310]  
专题中国科学院上海生命科学研究院营养科学研究所
作者单位1.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci, State Key Lab Mol Biol, Shanghai 200031, Peoples R China;
2.Univ Chinese Acad Sci, Shanghai 200031, Peoples R China;
3.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci, Shanghai Key Lab Mol Androl, Shanghai, Peoples R China;
4.Chinese Acad Sci, Shanghai Inst Biol Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Inst Neurosci,State Key Lab Neurosci, Shanghai, Peoples R China;
5.Chinese Acad Sci, Shanghai Inst Biol Sci, CAS MPG Partner Inst Computat Biol, Key Lab Computat Biol, Shanghai, Peoples R China,
推荐引用方式
GB/T 7714		 Zhang, Fengjuan,Zhang, Ying,Lv, Xiaolong,et al. Evolution of an X-Linked miRNA Family Predominantly Expressed in Mammalian Male Germ Cells[J]. MOLECULAR BIOLOGY AND EVOLUTION,2019,36(4):663-678.
APA Zhang, Fengjuan.,Zhang, Ying.,Lv, Xiaolong.,Xu, Beiying.,Zhang, Hongdao.,...&,.(2019).Evolution of an X-Linked miRNA Family Predominantly Expressed in Mammalian Male Germ Cells.MOLECULAR BIOLOGY AND EVOLUTION,36(4),663-678.
MLA Zhang, Fengjuan,et al."Evolution of an X-Linked miRNA Family Predominantly Expressed in Mammalian Male Germ Cells".MOLECULAR BIOLOGY AND EVOLUTION 36.4(2019):663-678.

入库方式： OAI收割

来源：上海营养与健康研究所

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