中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Cloning, expression, and characterization of a novel superoxide dismutase from deep-sea sea cucumber

文献类型:期刊论文

作者Li, Yanan2,4; Yan, Lei3; Kong, Xue1; Chen, Jiawei4; Zhang, Haibin4
刊名INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
出版日期2020-11-15
卷号163页码:1875-1883
ISSN号0141-8130
关键词Purification Deep-sea enzyme The Mariana trench
DOI10.1016/j.ijbiomac.2020.09.135
英文摘要Superoxide dismutases (SODs) are the first line of defense against oxidative damage caused by reactive oxygen species and are associated with stress tolerance. Accordingly, many studies have focused on SODs, especially those from extreme habitats. In this study, we reported a novel Cu,Zn-SOD from the new species Benthodytes marianensis sp. nov (hereafter denoted as Bm-Cu,Zn-SOD) collected from the Mariana Trench. The purified recombinant enzyme with an approx. Mr. of 38 kDa was intracellular and dimeric, and it expressed activity at an optimal temperature of 30 degrees C and optimum pH of 7.4. The Km and Vmax values of Bm-Cu,Zn-SOD were 0.046 +/- 0.004 mM and 1209.151 +/- 24.057 U/mg, respectively. Under the tested conditions, Bm-Cu,Zn-SOD resisted interference from chemicals (beta-mercaptoethanol and ethylenediaminetetraacetic acid), denaturants (urea and guanidine hydrochloride), and high salinity, showing high kinetic stability. Furthermore, Bm-Cu,Zn-SOD could be activated by high hydrostatic pressure. All these properties suggested adaptation to the deep-sea environment and thus indicated its potential future applications. (C) 2020 Elsevier B.V. All rights reserved.
WOS关键词DIFFERENTIAL EXPRESSION ; MOLECULAR-CLONING ; COPPER ; GENE ; HOLOTHURIANS ; TEMPERATURE ; CUZNSOD
资助项目National Key Research and Development Program of China[2018YFC0309804] ; National Key Research and Development Program of China[2016YFC0304905] ; Strategic Priority Research Program of the Chinese Academy of Sciences (CAS)[XDA22040502] ; Sanya Key Laboratory Program[L1501] ; Key Laboratory of Open-Sea Fishery Development, Ministry of Agriculture and Rural Affairs of the People's Republic of China
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种英语
出版者ELSEVIER
WOS记录号WOS:000579839600186
资助机构National Key Research and Development Program of China ; Strategic Priority Research Program of the Chinese Academy of Sciences (CAS) ; Sanya Key Laboratory Program ; Key Laboratory of Open-Sea Fishery Development, Ministry of Agriculture and Rural Affairs of the People's Republic of China
源URL[http://ir.idsse.ac.cn/handle/183446/8019]  
专题研究生部
深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组
通讯作者Zhang, Haibin
作者单位1.Jiangsu Ocean Univ, Jiangsu Key Lab Marine Bioresources & Ecoenvironm, Lianyungang 222005, Peoples R China
2.Zhongkai Univ Agr & Engn, Coll Anim Sci & Technol, Guangzhou 510225, Peoples R China
3.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab Open Sea Fishery Dev, Minist Agr & Rural Affairs Peoples Republ China, Guangzhou 510300, Peoples R China
4.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
推荐引用方式
GB/T 7714
Li, Yanan,Yan, Lei,Kong, Xue,et al. Cloning, expression, and characterization of a novel superoxide dismutase from deep-sea sea cucumber[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2020,163:1875-1883.
APA Li, Yanan,Yan, Lei,Kong, Xue,Chen, Jiawei,&Zhang, Haibin.(2020).Cloning, expression, and characterization of a novel superoxide dismutase from deep-sea sea cucumber.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,163,1875-1883.
MLA Li, Yanan,et al."Cloning, expression, and characterization of a novel superoxide dismutase from deep-sea sea cucumber".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 163(2020):1875-1883.

入库方式: OAI收割

来源:深海科学与工程研究所

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