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Heat shock transcription factor 1 regulates the fetal gamma-globin expression in a stress-dependent and independent manner during erythroid differentiation

文献类型：期刊论文

作者Gao, Jie1,4,5; Liu, Jinhua1,4,5; Zhang, Lingling3; Zhang, Yingnan1,4,5; Guo, Qing1,4,5; Li, Yapu1,4,5; Tong, Jingyuan1,4,5; Wang, Hongtao1,4,5; Zhou, Jiaxi1,4,5; Zhu, Fan2
刊名EXPERIMENTAL CELL RESEARCH
出版日期2020-02-15
卷号387期号:2页码:10
关键词HSF1 beta-globin genes Transcription regulation Stress
ISSN号0014-4827
DOI10.1016/j.yexcr.2019.111780
通讯作者Shi, Lihong(shilihongxys@ihcams.ac.cn) ; Zhao, Hui(zhaohui@tjcu.edu.cn)
英文摘要Heat shock transcription factor 1 (HSF1) is a highly versatile transcription factor that, in addition to protecting cells against proteotoxic stress, is also critical during diverse developmental processes. Although the functions of HSF1 have received considerable attention, its potential role in beta-globin gene regulation during erythropoiesis has not been fully elucidated. Here, after comparing the transcriptomes of erythrocytes differentiated from cord blood or adult peripheral blood hematopoietic progenitor CD34(+) cells in vitro, we constructed the molecular regulatory network associated with beta-globin genes and identified novel and putative globin gene regulators by combining the weighted gene coexpression network analysis (WGCNA) and context likelihood of relatedness (CLR) algorithms. Further investigation revealed that one of the identified regulators, HSF1, acts as a key activator of the gamma-globin gene in human primary erythroid cells in both erythroid developmental stages. While during stress, HSF1 is required for heat-induced globin gene activation, and HSF1 downregulation markedly decreases globin gene induction in K562 cells. Mechanistically, HSF1 occupies DNase I hypersensitive site 3 of the locus control region upstream of beta-globin genes via its canonical binding motif. Hence, HSF1 executes stress-dependent and -independent roles in fetal gamma-globin regulation during erythroid differentiation.
资助项目National Key Research and Development Program of China[2016YFA0102300] ; National Key Research and Development Program of China[2017YFA0103100] ; National Key Research and Development Program of China[2017YFA0103102] ; CAMS Innovation Fund for Medical Sciences (CIFMS)[2016-I2M-3-002] ; CAMS Innovation Fund for Medical Sciences (CIFMS)[2016-I2M-1-018] ; CAMS Innovation Fund for Medical Sciences (CIFMS)[2017-I2M-1-015] ; National Natural Science Foundation of China[81870089] ; National Natural Science Foundation of China[81700105] ; National Natural Science Foundation of China[81890990] ; National Natural Science Foundation of China[61802360] ; Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences[2018PT31033] ; Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences[2019PT310022] ; Tianjin Municipal Science and Technology Commission[15JCYBJC54500]
WOS研究方向Oncology ; Cell Biology
语种英语
WOS记录号WOS:000512482300016
出版者ELSEVIER INC
源URL[http://119.78.100.138/handle/2HOD01W0/10550]  
专题中国科学院重庆绿色智能技术研究院
通讯作者Shi, Lihong; Zhao, Hui
作者单位1.Chinese Acad Med Sci, Ctr Stem Cell Med, Beijing 100730, Peoples R China
2.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing, Peoples R China
3.Tianjin Univ Commerce, Sch Biotechnol & Food Sci, Tianjin Key Lab Food & Biotechnol, Tianjin 300134, Peoples R China
4.Chinese Acad Med Sci & Peking Union Med Coll, Inst Hematol, Natl Clin Res Ctr Blood Dis, State Key Lab Expt Hematol, Tianjin 300020, Peoples R China
5.Chinese Acad Med Sci & Peking Union Med Coll, Blood Dis Hosp, Tianjin 300020, Peoples R China
推荐引用方式
GB/T 7714		 Gao, Jie,Liu, Jinhua,Zhang, Lingling,et al. Heat shock transcription factor 1 regulates the fetal gamma-globin expression in a stress-dependent and independent manner during erythroid differentiation[J]. EXPERIMENTAL CELL RESEARCH,2020,387(2):10.
APA Gao, Jie.,Liu, Jinhua.,Zhang, Lingling.,Zhang, Yingnan.,Guo, Qing.,...&Zhao, Hui.(2020).Heat shock transcription factor 1 regulates the fetal gamma-globin expression in a stress-dependent and independent manner during erythroid differentiation.EXPERIMENTAL CELL RESEARCH,387(2),10.
MLA Gao, Jie,et al."Heat shock transcription factor 1 regulates the fetal gamma-globin expression in a stress-dependent and independent manner during erythroid differentiation".EXPERIMENTAL CELL RESEARCH 387.2(2020):10.

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来源：重庆绿色智能技术研究院

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