A self-assembled nanocompartment in anammox bacteria for resisting intracelluar hydroxylamine stress
文献类型：期刊论文
| 作者 | Xing, Chong-Yang1,2; Fan, Yu-Chen4; Chen, Xuan4; Guo, Jin-Song4 ; Shen, Yu3; Yan, Peng4; Fang, Fang4; Chen, You-Peng1,2,4
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| 刊名 | SCIENCE OF THE TOTAL ENVIRONMENT
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| 出版日期 | 2020-05-15 |
| 卷号 | 717页码:8 |
| 关键词 | Anammox Nanocompartment Hydroxylamine Hydroxylamine oxidoreductase Encapsulin shell protein |
| ISSN号 | 0048-9697 |
| DOI | 10.1016/j.scitotenv.2020.137030 |
| 通讯作者 | Chen, You-Peng(ypchen@cigit.ac.cn) |
| 英文摘要 | Anammox bacteria play an important role in the global nitrogen cycle, but research on anammoxosome structure is still in its initial stages. In particular, the anammox bacteria genome contains nanocompartments gene loci. However, the function and structure of the nanocompartments in anammox bacteria is poorly understood. We apply genetic engineering to demonstrate the self-assembled nanocompartments of anammox bacteria. The encapsulin shell protein (cEnc) and cargo protein hydroxylamine oxidoreductase (HAO) can self-assemble to form regular nanocompartments (about 128 nm in diameter) in vitro. Cell growth curve tests show that nanocompartments help model bacteria resist hydroxylamine (NH2OH) stress. Batch test results and transcriptional data show that cEnc and HAO are highly expressed in response to the negative effects of NH2OH on anammox efficiency, predicting a potential role of nanocompartments in helping anammox bacteria resist NH2OH stress. These findings improve our understanding of the mechanisms by which anammox bacteria respond to harmful environmental metabolites. (C) 2020 Elsevier B.V. All rights reserved. |
| 资助项目 | National Natural Science Foundation of China[21876016] ; National Natural Science Foundation of China[51878091] ; Chongqing Science and Technology Bureau[cstc2018jcyjAX0366] ; Chongqing Science and Technology Bureau[cstc2018jcyjAX0638] ; Fundamental Research Funds for the Central Universities[2019CDCGHS311] |
| WOS研究方向 | Environmental Sciences & Ecology |
| 语种 | 英语 |
| WOS记录号 | WOS:000519994800013 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/10735]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Chen, You-Peng |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Key Lab Reservoir Aquat Environm, Chongqing Inst Green & Intelligence Technol, Chongqing 400714, Peoples R China 3.Chongqing Technol & Business Univ, Cooperat Intelligent Mfg Serv, Natl Base Int Sci & Technol, Chongqing 400067, Peoples R China 4.Chongqing Univ, Key Lab Three Gorges Reservoir Reg Ecoenvironm MO, Chongqing 400045, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xing, Chong-Yang,Fan, Yu-Chen,Chen, Xuan,et al. A self-assembled nanocompartment in anammox bacteria for resisting intracelluar hydroxylamine stress[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2020,717:8. |
| APA | Xing, Chong-Yang.,Fan, Yu-Chen.,Chen, Xuan.,Guo, Jin-Song.,Shen, Yu.,...&Chen, You-Peng.(2020).A self-assembled nanocompartment in anammox bacteria for resisting intracelluar hydroxylamine stress.SCIENCE OF THE TOTAL ENVIRONMENT,717,8. |
| MLA | Xing, Chong-Yang,et al."A self-assembled nanocompartment in anammox bacteria for resisting intracelluar hydroxylamine stress".SCIENCE OF THE TOTAL ENVIRONMENT 717(2020):8. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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