Enriching the Production of 2-Methyl-1-Butanol in Fermentation Process Using Corynebacterium crenatum
文献类型：期刊论文
| 作者 | Su, HaiFeng2,3 ; Chen, Hua1; Lin, JiaFu4
|
| 刊名 | CURRENT MICROBIOLOGY
 |
| 出版日期 | 2020-04-16 |
| 页码 | 8 |
| ISSN号 | 0343-8651 |
| DOI | 10.1007/s00284-020-01961-0 |
| 通讯作者 | Lin, JiaFu(linjiafu@cdu.edu.cn) |
| 英文摘要 | Non-natural 2-methyl-1-butanol (2 MB) has been biosynthesized through the modification of metabolic pathways using Corynebacterium crenatum, a non-model host. However, its production capacity is not effectively improved. In this study, the fermentation process was strengthened through factor combination design (FCD) for enhancing the production of 2 MB. Our results showed that the highest production of 2 MB, 3-methyl-1-butanol (3 MB), ethanol, and total solvent was 4.87 +/- 0.39 g/L, 3.57 +/- 0.21 g/L, 5.74 +/- 0.43 g/L, and 14.18 g/L, respectively, under the optimal fermentation conditions. The optimal fermentation conditions were determined through the FCD to be as follows: pH of 6.5, IPTG concentration of 1.2 mM, fermentation temperature of 32 degrees C, and fermentation time of 96 h. This study provides a significant guidance for the optimal control technology of the genetically engineered C. crenatum, and also a useful reference for the industrial production of 2 MB via the microbial fermentation approach. |
| 资助项目 | Open Project Program of the State Key Laboratory of Petroleum Pollution Control[PPC2017004] ; CNPC Research Institute of Safety and Environmental Technology ; National Natural Science Foundation[cstc2017jcyjA02138] ; frontier research program of Chongqing[cstc2017jcyjA02138] ; Chongqing Science Committee, Key laboratory of Degraded and Unused Land Consolidation Engineering, the Ministry of Natural and Resources[SXDJ2019] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000533859200004 |
| 出版者 | SPRINGER |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/11019]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Lin, JiaFu |
| 作者单位 | 1.Zunyi Inst Forestry Sience, Zunyi 563002, Guizhou, Peoples R China 2.State Key Lab Petr Pollut Control, Beijing 102206, Peoples R China 3.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Key Lab Reservoir Water Environm, 266 Fangzheng Ave,Shuitu High Tech Pk, Chongqing 400714, Peoples R China 4.Chengdu Univ, Sichuan Ind Inst Antibiot, Antibiot Res & Re Evaluat Key Lab Sichuan Prov, Chengdu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Su, HaiFeng,Chen, Hua,Lin, JiaFu. Enriching the Production of 2-Methyl-1-Butanol in Fermentation Process Using Corynebacterium crenatum[J]. CURRENT MICROBIOLOGY,2020:8. |
| APA | Su, HaiFeng,Chen, Hua,&Lin, JiaFu.(2020).Enriching the Production of 2-Methyl-1-Butanol in Fermentation Process Using Corynebacterium crenatum.CURRENT MICROBIOLOGY,8. |
| MLA | Su, HaiFeng,et al."Enriching the Production of 2-Methyl-1-Butanol in Fermentation Process Using Corynebacterium crenatum".CURRENT MICROBIOLOGY (2020):8. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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