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Label-free single-molecule identification of telomere G-quadruplexes with a solid-state nanopore sensor

文献类型：期刊论文

作者Wang, Sen3,4; Liang, Liyuan3,4; Tang, Jing3,4; Cai, Yao1,3; Zhao, Chuanqi2,4; Fang, Shaoxi3,4; Wang, Huabin3,4; Weng, Ting3,4; Wang, Liang3,4; Wang, Deqiang3,4
刊名RSC ADVANCES
出版日期2020-07-20
卷号10期号:45页码:27215-27224
DOI10.1039/d0ra05083k
通讯作者Liang, Liyuan(liangliyuan@cigit.ac.cn) ; Wang, Deqiang(dqwang@cigit.ac.cn)
英文摘要Telomere sequences can spontaneously form G-quadruplexes (G4) in the presence of some cations. In view of their relevance to genetic processes and potential as therapeutic-targets, hitherto, a wealth of conventional techniques have been reported for interrogation of G4 conformation diversity and corresponding folding kinetics, most of which are limited in precision and sensitivity. This work introduces a label-free solid-state nanopore (SSN) approach for the determination of inter-, intra- and tandem molecular G4 with distinct base permutation in various cation buffers with a tailored aperture and meanwhile captures the single-molecule G4 folding process. SSN translocation properties elucidated that both inter- and intramolecular G4 generated higher current blockage with longer duration than flexible homopolymer nucleotide, and intramolecular G4 are structurally more stable with higher event frequency and longer blockage time than intermolecular ones; base arrangement played weak role in translocation behaviors; the same sequences with one, two and three G4 skeletons displayed an increase in current blockage and a gradual extension in dwell time with the increase of molecule size recorded in the same nanopore. We observed the conformation change of single-molecule G4 which indicated the existence of folding/unfolding equilibration in nanopore, and real-time test suggested a gradual formation of G4 with time. Our results could provide a continued and improved understanding of the underlying relevance of structural stability and G4 folding process by utilizing SSN platform which exhibits strategic potential advances over the other methods with high spatial and temporal resolution.
资助项目Natural Science Foundation of Chongqing, China[cstc2017jcyjB0105] ; Youth Innovation Promotion Association[2017392] ; Pioneer Hundred Talents Program of the Chinese Academy of Sciences ; Instrumentation Development Program of the Chinese Academy of Sciences[YZ201568] ; University of Chinese Academy of Sciences
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000555612200062
出版者ROYAL SOC CHEMISTRY
源URL[http://119.78.100.138/handle/2HOD01W0/11654]  
专题中国科学院重庆绿色智能技术研究院
通讯作者Liang, Liyuan; Wang, Deqiang
作者单位1.Jilin Univ, Coll Instrument Sci & Elect Engn, Changchun 130016, Peoples R China
2.Chinese Acad Sci, Changchun Inst Appl Chem, Lab Chem Biol, State Key Lab Rare Earth Resource Utilizat, Changchun 130022, Jilin, Peoples R China
3.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing Key Lab Multiscale Mfg Technol, Chongqing 400714, Peoples R China
4.Univ Chinese Acad Sci, Chongqing Sch, Chongqing 400714, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Sen,Liang, Liyuan,Tang, Jing,et al. Label-free single-molecule identification of telomere G-quadruplexes with a solid-state nanopore sensor[J]. RSC ADVANCES,2020,10(45):27215-27224.
APA Wang, Sen.,Liang, Liyuan.,Tang, Jing.,Cai, Yao.,Zhao, Chuanqi.,...&Wang, Deqiang.(2020).Label-free single-molecule identification of telomere G-quadruplexes with a solid-state nanopore sensor.RSC ADVANCES,10(45),27215-27224.
MLA Wang, Sen,et al."Label-free single-molecule identification of telomere G-quadruplexes with a solid-state nanopore sensor".RSC ADVANCES 10.45(2020):27215-27224.

入库方式： OAI收割

来源：重庆绿色智能技术研究院

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