Detection of DNA, RNA Bases and Rare Bases by Terahertz Spectroscopy
文献类型：期刊论文
| 作者 | Tang Ming-jie1,2 ; Yan Shi-han1; Zhang Ming-kun1; Wei Dong-shan1; Du Chun-lei1; Cui Hong-liang1
|
| 刊名 | SPECTROSCOPY AND SPECTRAL ANALYSIS
 |
| 出版日期 | 2020-08-01 |
| 卷号 | 40期号:8页码:2388-2391 |
| 关键词 | Base Rare base Terahertz spectroscopy Raman spectroscopy |
| ISSN号 | 1000-0593 |
| DOI | 10.3964/j.issn.1000-0593(2020)08-2388-04 |
| 通讯作者 | Du Chun-lei() |
| 英文摘要 | Spectral analysis of nucleic acid molecular bases is of great significance to the study of biogenetics. In this paper, the feasibility of terahertz time domain spectroscopy (THz-TDS) and Raman spectroscopy for the spectroscopic detection of DNA, RNA bases and rare bases is discussed. The terahertz and Raman spectra of seven solid nucleic acid bases were analyzed, and the THz-TDS and Raman spectroscopy techniques were compared. In THz-TDS experiments, cytosine (C), guanine (G) , adenine (A) , thymine (T, DNA-specific) and uracil (U, RNA-specific) and rare bases (5-methylcytosine ( m(5)C), 1-methyladenine (m(1)A)) were identified. Signature absorption peaks and absorption intensities differ significantly in the 0. 2 similar to 2. 0 THz, and the differences of seven bases can be identified intuitively; In Raman spectroscopy, the seven bases also show many distinct characteristic peaks, however the characteristic peaks of Raman spectroscopy are complex and many, so it is not intuitive to identify many substances. The difference of absorption intensity is related to the thickness of powder, particle size and the depth of light focusing. The fluorescence of samples also interfere with Raman spectra. The laser may also damage biological samples. The experimental results show that these two technologies can identify seven common and rare bases. THz-TDS technology is superior to Raman spectroscopy in the ability to identify these seven bases, showing a relatively concise, fast and non-destructive detection performance. THz-TDS technology not only provides a fast and accurate method for the identification of DNA, RNA and rare bases, but also lays an experimental foundation for the study of biogenetics. |
| WOS研究方向 | Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000564304300010 |
| 出版者 | OFFICE SPECTROSCOPY & SPECTRAL ANALYSIS |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/11702]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Du Chun-lei |
| 作者单位 | 1.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing Key Lab Multiscale Mfg Technol, Chongqing 400714, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tang Ming-jie,Yan Shi-han,Zhang Ming-kun,et al. Detection of DNA, RNA Bases and Rare Bases by Terahertz Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2020,40(8):2388-2391. |
| APA | Tang Ming-jie,Yan Shi-han,Zhang Ming-kun,Wei Dong-shan,Du Chun-lei,&Cui Hong-liang.(2020).Detection of DNA, RNA Bases and Rare Bases by Terahertz Spectroscopy.SPECTROSCOPY AND SPECTRAL ANALYSIS,40(8),2388-2391. |
| MLA | Tang Ming-jie,et al."Detection of DNA, RNA Bases and Rare Bases by Terahertz Spectroscopy".SPECTROSCOPY AND SPECTRAL ANALYSIS 40.8(2020):2388-2391. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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