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Detection of gene mutation responsible for Huntington's disease by terahertz attenuated total reflection microfluidic spectroscopy

文献类型：期刊论文

作者Tang, Mingjie1,2; Zhang, Mingkun1; Xia, Liangping1,3; Wei, Dongshan1,4; Yang, Zhongbo1; Yan, Shihan1; Du, Chunlei1; Cui, Hong-Liang1
刊名JOURNAL OF BIOPHOTONICS
出版日期2020-10-11
页码10
关键词ATR DNA oligonucleotides microfluidic cell repeated sequence terahertz absorption
ISSN号1864-063X
DOI10.1002/jbio.202000315
通讯作者Xia, Liangping(xialp@yznu.edu.cn) ; Cui, Hong-Liang(hcui@cigit.ac.cn)
英文摘要Terahertz absorption spectroscopy based on attenuated total reflection (ATR) from a microfluidic sample cell was designed and implemented to detect gene mutations leading to Huntington's disease (HD). The self-developed compact ATR microfluidic system was employed to detect two groups of base-repeated DNA molecules combined with a terahertz time-domain spectrometer in a marker-free manner. The first group featured different repetition patterns of oligonucleotide fragments, and the second group included the HD gene. For the oligonucleotides of different repetition patterns, there were significant differences among the three oligonucleotides with three repeats of the double bases, which could be unambiguously classified and identified; For the HD gene, it was found that the magnitude of the terahertz absorption coefficients of the four oligonucleotide solutions was, in ascending order, CAG-4 < CAG-16 < CAG-32 < CAG-40 (the numbers are the repeat times of the CAG base segment, with 40 repeats belonging to the HD gene), when the concentration of oligonucleotide was 1 mg/mL. Principal component analysis result indicated that the spectral differences of the four oligonucleotide solutions with different CAG repeat times were statistically significant and clearly distinguishable. These results demonstrate the potential of terahertz spectroscopy as a noninvasive, unmarked, fast and low-cost assay for gene diagnosis and clinical disease detection.
资助项目Central Government Supported Key Instrument Program of China[YXGYQ201700136] ; National Key Research and Development Program of China[2017YFF0106303] ; National Natural Science Foundation of China[61775213 61875196] ; Natural Science Foundation of Chongqing[cstc2019jcyj-msxmX0654 cstc2019jcyj-msxmX0352]
WOS研究方向Biochemistry & Molecular Biology ; Biophysics ; Optics
语种英语
WOS记录号WOS:000578419600001
出版者WILEY-V C H VERLAG GMBH
源URL[http://119.78.100.138/handle/2HOD01W0/11889]  
专题中国科学院重庆绿色智能技术研究院
通讯作者Xia, Liangping; Cui, Hong-Liang
作者单位1.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Yangtze Normal Univ, Key Lab Micro Nano Optoelect Devices & Intelligen, Chongqing, Peoples R China
4.Dongguan Univ Technol, Sch Elect Engn, Dongguan, Peoples R China
推荐引用方式
GB/T 7714		 Tang, Mingjie,Zhang, Mingkun,Xia, Liangping,et al. Detection of gene mutation responsible for Huntington's disease by terahertz attenuated total reflection microfluidic spectroscopy[J]. JOURNAL OF BIOPHOTONICS,2020:10.
APA Tang, Mingjie.,Zhang, Mingkun.,Xia, Liangping.,Wei, Dongshan.,Yang, Zhongbo.,...&Cui, Hong-Liang.(2020).Detection of gene mutation responsible for Huntington's disease by terahertz attenuated total reflection microfluidic spectroscopy.JOURNAL OF BIOPHOTONICS,10.
MLA Tang, Mingjie,et al."Detection of gene mutation responsible for Huntington's disease by terahertz attenuated total reflection microfluidic spectroscopy".JOURNAL OF BIOPHOTONICS (2020):10.

入库方式： OAI收割

来源：重庆绿色智能技术研究院

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