Reduction chemistry-assisted nanopore determination method for immunoglobulin isotypes
文献类型：期刊论文
| 作者 | Liu, Qianshan1,2; Wang, Yunjiao1,2 ; Liu, Yaqing2; Wang, Han1,2; Li, Wei1,2; Tang, Peng2; Weng, Tin2; Zhou, Shuo2; Liang, Liyuan1,2; Yuan, Jiahu1,2
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| 刊名 | NANOSCALE
 |
| 出版日期 | 2020-10-14 |
| 卷号 | 12期号:38页码:19711-19718 |
| ISSN号 | 2040-3364 |
| DOI | 10.1039/d0nr04900j |
| 通讯作者 | Yuan, Jiahu() ; Wang, Deqiang(dqwang@cigit.ac.cn) ; Wang, Lang(yjh@cigit.ac.cn) |
| 英文摘要 | Immunoglobulins can bind to an unlimited array of foreign antigens presented to the immune system. Among those isotypes, IgG and IgM play crucial roles in initial immune defense associated with innate immunity factors. Hence, the determination of IgG and IgM deficiencies or varying concentrations is widely used as a diagnostic indicator for immune deficiency disorders. Herein, we report a reduction chemistry-assisted nanopore method for IgG and IgM determination. TCEP (tris(2-carboxyethyl)phosphine) was used to cleave Ig proteins in fragments by means of disulfide bond reduction under different experimental conditions. This strategy enabled the observation of distinguishable current signals afforded by separated polypeptide fragments in an alpha HL nanopore. Together with molecular dynamics (MD) simulation results, highly effective electrostatic potentials and H-bonds, the dominant factors for these current signals, facilitated the capture of Ig fragments in an alpha-HL nanopore. More importantly, the signature signals were applicable for differentiating between IgG and IgM in blood serum without any problems of protein adsorption and clogging in the nanopore sensing. Furthermore, with comparative sensing sensitivity and selectivity, it is concluded that our method is a label-free single-molecule approach to measuring disease states that present as a result of the absence or over presence of immunoglobulin isotypes. |
| 资助项目 | National Natural Science Foundation of China[31800711] ; Natural Science Foundation of Chongqing, China[cstc2018jcyjAX0304] ; Entrepreneurship and Innovation Support Program for Returned Oversea Students of Chongqing, China[cx2018156] ; Youth Innovation Promotion Association of CAS[2017392] ; [CQYC201905007] |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:000578100000016 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/11892]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Yuan, Jiahu; Wang, Deqiang; Wang, Lang |
| 作者单位 | 1.Univ Chinese Acad Sci, Chongqing Sch, Chongqing 400714, Peoples R China 2.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Qianshan,Wang, Yunjiao,Liu, Yaqing,et al. Reduction chemistry-assisted nanopore determination method for immunoglobulin isotypes[J]. NANOSCALE,2020,12(38):19711-19718. |
| APA | Liu, Qianshan.,Wang, Yunjiao.,Liu, Yaqing.,Wang, Han.,Li, Wei.,...&Wang, Lang.(2020).Reduction chemistry-assisted nanopore determination method for immunoglobulin isotypes.NANOSCALE,12(38),19711-19718. |
| MLA | Liu, Qianshan,et al."Reduction chemistry-assisted nanopore determination method for immunoglobulin isotypes".NANOSCALE 12.38(2020):19711-19718. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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