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Detection of single-base mutation of DNA oligonucleotides with different lengths by terahertz attenuated total reflection microfluidic cell

文献类型：期刊论文

作者Tang, Mingjie3,4; Zhang, Mingkun4; Xia, Liangping2,4; Yang, Zhongbo4; Yan, Shihan4; Wang, Huabin4; Wei, Dongshan1,4; Du, Chunlei4; Cui, Hong-Liang4
刊名BIOMEDICAL OPTICS EXPRESS
出版日期2020-09-01
卷号11期号:9页码:5362-5372
ISSN号2156-7085
DOI10.1364/BOE.400487
通讯作者Wei, Dongshan(dswei@dgut.edu.cn)
英文摘要Many human genetic diseases are caused by single-base mutation in the gene sequence. Since DNA molecules with single-base mutation are extremely difficult to differentiate, existing detection methods are invariably complex and time-consuming. We propose a new label-free and fast terahertz (THz) spectroscopic technique based on a home-made terahertz attenuated total reflection (ATR) microfluidic cell and a terahertz time-domain spectroscopy (THz-TDS) system to detect single-base-mutated DNA molecules. The detected object DNA molecules are normal hemoglobin gene, sickle cell anemia gene (15 nt), JAK2 gene wild type and JAK2 V617F gene mutation (39 nt) from sickle cell anemia and thrombocytopenia, respectively. Results show that the oligonucleotide fragments with single-base mutation can be identified by THz spectroscopy combined with the ATR microfluidic cell, and the recognition effect of short oligonucleotide fragments with single-base mutation is better than that of long oligonucleotide fragments. The terahertz biosensor is shown to have high sensitivity and can be used to detect DNA molecules directly in the solution environment. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
资助项目National Natural Science Foundation of China[61771138] ; National Natural Science Foundation of China[61775213] ; National Natural Science Foundation of China[61875196]
WOS研究方向Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
语种英语
WOS记录号WOS:000577455500036
出版者OPTICAL SOC AMER
源URL[http://119.78.100.138/handle/2HOD01W0/11907]  
专题中国科学院重庆绿色智能技术研究院
通讯作者Wei, Dongshan
作者单位1.Dongguan Univ Technol, Sch Elect Engn, Dongguan 523808, Guangdong, Peoples R China
2.Yangtze Normal Univ, Key Lab Micro Nano Optoelect Devices & Intelligen, Chongqing 408100, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China
推荐引用方式
GB/T 7714		 Tang, Mingjie,Zhang, Mingkun,Xia, Liangping,et al. Detection of single-base mutation of DNA oligonucleotides with different lengths by terahertz attenuated total reflection microfluidic cell[J]. BIOMEDICAL OPTICS EXPRESS,2020,11(9):5362-5372.
APA Tang, Mingjie.,Zhang, Mingkun.,Xia, Liangping.,Yang, Zhongbo.,Yan, Shihan.,...&Cui, Hong-Liang.(2020).Detection of single-base mutation of DNA oligonucleotides with different lengths by terahertz attenuated total reflection microfluidic cell.BIOMEDICAL OPTICS EXPRESS,11(9),5362-5372.
MLA Tang, Mingjie,et al."Detection of single-base mutation of DNA oligonucleotides with different lengths by terahertz attenuated total reflection microfluidic cell".BIOMEDICAL OPTICS EXPRESS 11.9(2020):5362-5372.

入库方式： OAI收割

来源：重庆绿色智能技术研究院

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