Global mapping of live cell mechanical features usingPeakForce QNM AFM
文献类型：期刊论文
| 作者 | Hu JR(胡锦荣) ; Chen SB(陈深宝); Huang DD(黄丹丹); Zhang Y(章燕); Lv SQ(吕守芹); Long M(龙勉)
|
| 刊名 | Biophysics Reports
 |
| 出版日期 | 2020-01-22 |
| 卷号 | 6期号:1页码:9-18 |
| DOI | 10.1007/s41048-019-00103-9 |
| 英文摘要 | Atomic force microscopy (AFM) has been widely used to acquire surface topography upon different scanning modes and to quantify mechanical properties of a cell using single-point ramp force mode. However, these traditional measurements need massive force curves originating from multiple points of a cell to exclude the potential errors resulted from limited and factitious selections of testing points, making the measurements time-consuming and highly localized. PeakForce Quantitative NanoMechanics (PF QNM) is a high-speed (faster by 3–4 order of magnitude) and global surface mechanical property mapping method with high spatial resolution, overcoming the drawbacks of traditional ramp mode especially used for a live cell with high heterogeneity. In this protocol, we elaborated how to run PF QNM measurements for live cells and relevant modification may be needed when extending this method to other cell-like soft materials. |
| 分类号 | 其他 |
| 语种 | 英语 |
| 其他责任者 | Shouqin Lü, Mian Long |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/85684]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Hu JR,Chen SB,Huang DD,et al. Global mapping of live cell mechanical features usingPeakForce QNM AFM[J]. Biophysics Reports,2020,6(1):9-18. |
| APA | 胡锦荣,陈深宝,黄丹丹,章燕,吕守芹,&龙勉.(2020).Global mapping of live cell mechanical features usingPeakForce QNM AFM.Biophysics Reports,6(1),9-18. |
| MLA | 胡锦荣,et al."Global mapping of live cell mechanical features usingPeakForce QNM AFM".Biophysics Reports 6.1(2020):9-18. |
入库方式： OAI收割
来源：力学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。