Speeding up selenite bioremediation using the highly selenite-tolerant strain Providencia rettgeri HF16-A novel mechanism of selenite reduction based on proteomic analysis
文献类型：期刊论文
| 作者 | Huang, ShengWei1,2 ; Wang, Yuting3,4; Tang, Caiguo1,2; Jia, HuiLing1,5; Wu, Lifang1,2,5
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| 刊名 | JOURNAL OF HAZARDOUS MATERIALS
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| 出版日期 | 2021-03-15 |
| 卷号 | 406 |
| ISSN号 | 0304-3894 |
| 关键词 | Providencia rettgeri HF16 Selenite reduction Biogenic selenium nanoparticles Proteomics Sulfite reductase |
| DOI | 10.1016/j.jhazmat.2020.124690 |
| 通讯作者 | Wu, Lifang(lfwu@ipp.ac.cn) |
| 英文摘要 | Selenite in the environment is extremely biotoxic, thus, the biotransformation of selenite into selenium nanoparticles (SeNPs) by microorganisms is gaining increasing interest. However, the relatively low selenite tolerance and slow processing by known microorganisms limit its application. In this study, a highly selenite-resistant strain (up to 800 mM) was isolated from coalmine soil and identified as Providencia rettgeri HF16. Remarkably, 5 mM selenite was entirely transformed by this strain within 24 h, and SeNPs were detected as early as 2 h of incubation, which is a more rapid conversion than that described for other microorganisms. The SeNPs were spherical in shape with diameters ranging from 120 nm to 295 nm, depending on the incubation time. Moreover, in vitro selenite-reduction activity was detected in the cytoplasmic protein fraction with NADPH or NADH serving as electron donors. Proteomics analysis and key enzyme activity tests revealed the presence of a sulfite reductase-mediated selenite reduction pathway. To our knowledge, this is the first report to identify the involvement of sulfite reductase in selenite reduction under physiological conditions. P. rettgeri HF16 could be a suitable and robust biocatalyst for the bioremediation of selenite, and would accelerate the efficient and economical synthesis of selenium nanoparticles. |
| 资助项目 | Natural Science Foundation of Anhui Province[2008085MC60] ; National Key R&D Program of China[2018YFD0502001] ; Science and Technology Service Program of Chinese Academy of Sciences[KFJ-STS-ZDTP-054] ; National Natural Science Foundation of China[31500531] ; Opening foundation of Anhui Province of Key Laboratory of Environmental Toxicology and Pollution Control Technology[Y79AH72588] ; Key Program of 13th Five-Year Plan, Hefei Institutes of Physical Science, Chinese Academy of Sciences[kp-2017-21] |
| WOS研究方向 | Engineering ; Environmental Sciences & Ecology |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000614541200003 |
| 资助机构 | Natural Science Foundation of Anhui Province ; National Key R&D Program of China ; Science and Technology Service Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; Opening foundation of Anhui Province of Key Laboratory of Environmental Toxicology and Pollution Control Technology ; Key Program of 13th Five-Year Plan, Hefei Institutes of Physical Science, Chinese Academy of Sciences |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/119626]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Wu, Lifang |
| 作者单位 | 1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Anhui, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab Environm Toxicol & Pollut Control Technol, Hefei 230026, Anhui, Peoples R China 3.Univ Sci & Technol China, Affiliated Hosp USTC 1, Div Life Sci & Med, Hefei 230001, Anhui, Peoples R China 4.Univ Sci & Technol China, Intelligent Pathol Inst, Div Life Sci & Med, Hefei 230036, Anhui, Peoples R China 5.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, ShengWei,Wang, Yuting,Tang, Caiguo,et al. Speeding up selenite bioremediation using the highly selenite-tolerant strain Providencia rettgeri HF16-A novel mechanism of selenite reduction based on proteomic analysis[J]. JOURNAL OF HAZARDOUS MATERIALS,2021,406. |
| APA | Huang, ShengWei,Wang, Yuting,Tang, Caiguo,Jia, HuiLing,&Wu, Lifang.(2021).Speeding up selenite bioremediation using the highly selenite-tolerant strain Providencia rettgeri HF16-A novel mechanism of selenite reduction based on proteomic analysis.JOURNAL OF HAZARDOUS MATERIALS,406. |
| MLA | Huang, ShengWei,et al."Speeding up selenite bioremediation using the highly selenite-tolerant strain Providencia rettgeri HF16-A novel mechanism of selenite reduction based on proteomic analysis".JOURNAL OF HAZARDOUS MATERIALS 406(2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
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