Senescence associated long non-coding RNA 1 regulates cigarette smoke-induced senescence of type II alveolar epithelial cells through sirtuin-1 signaling
文献类型：期刊论文
| 作者 | Yuan, Dong1,2; Liu, Yuanshun1; Li, Mengyu1,2; Zhou, Hongbin1; Cao, Liming1; Zhang, Xiaoqin1; Li, Yaqing1,3 |
| 刊名 | JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
 |
| 出版日期 | 2021-02-01 |
| 卷号 | 49 |
| 关键词 | Chronic obstructive pulmonary disease alveolar epithelial cells sirtuin-1 cellular senescence long non-coding RNA cigarette smoke |
| ISSN号 | 0300-0605 |
| DOI | 10.1177/0300060520986049 |
| 通讯作者 | Li, Yaqing(lidoctor03@126.com) |
| 英文摘要 | Objective The primary aim of our study was to explore the mechanisms through which long non-coding RNA (lncRNA)-mediated sirtuin-1 (SIRT1) signaling regulates type II alveolar epithelial cell (AECII) senescence induced by a cigarette smoke-media suspension (CSM). Methods Pharmacological SIRT1 activation was induced using SRT2104 and senescence-associated lncRNA 1 (SAL-RNA1) was overexpressed. The expression of SIRT1, FOXO3a, p53, p21, MMP-9, and TIMP-1 in different groups was detected by qRT-PCR and Western blotting; the activity of SA-beta gal was detected by staining; the binding of SIRT1 to FOXO3a and p53 gene transcription promoters was detected by Chip. Results We found that CSM increased AECII senescence, while SAL-RNA1 overexpression and SIRT1 activation significantly decreased levels of AECII senescence induced by CSM. Using chromatin immunoprecipitation, we found that SIRT1 bound differentially to transcriptional complexes on the FOXO3a and p53 promoters. Conclusion Our results suggested that lncRNA-SAL1-mediated SIRT1 signaling reduces senescence of AECIIs induced by CSM. These findings suggest a new therapeutic target to limit the irreversible apoptosis of lung epithelial cells in COPD patients. |
| 资助项目 | National Natural Science Foundation of China[81870028] ; National Natural Science Foundation of China[81470241] ; National Natural Science Foundation of China[81900029] ; Zhejiang Provincial Program for the Cultivation of High-Level Innovative Health Talents[A-2017-CXCR02] |
| WOS研究方向 | Research & Experimental Medicine ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000617326200001 |
| 出版者 | SAGE PUBLICATIONS LTD |
| 资助机构 | National Natural Science Foundation of China ; Zhejiang Provincial Program for the Cultivation of High-Level Innovative Health Talents |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/119641]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Li, Yaqing |
| 作者单位 | 1.Zhejiang Prov Peoples Hosp, Peoples Hosp, Dept Resp Med, Hangzhou Med Coll, Hangzhou, Zhejiang, Peoples R China 2.Bengbu Med Coll, Grad Dept, Bengbu, Anhui, Peoples R China 3.Univ Chinese Acad Sci, Dept Internal Med, Zhejiang Canc Hosp, Canc Hosp, 1 East Banshan Rd, Hangzhou 310022, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yuan, Dong,Liu, Yuanshun,Li, Mengyu,et al. Senescence associated long non-coding RNA 1 regulates cigarette smoke-induced senescence of type II alveolar epithelial cells through sirtuin-1 signaling[J]. JOURNAL OF INTERNATIONAL MEDICAL RESEARCH,2021,49. |
| APA | Yuan, Dong.,Liu, Yuanshun.,Li, Mengyu.,Zhou, Hongbin.,Cao, Liming.,...&Li, Yaqing.(2021).Senescence associated long non-coding RNA 1 regulates cigarette smoke-induced senescence of type II alveolar epithelial cells through sirtuin-1 signaling.JOURNAL OF INTERNATIONAL MEDICAL RESEARCH,49. |
| MLA | Yuan, Dong,et al."Senescence associated long non-coding RNA 1 regulates cigarette smoke-induced senescence of type II alveolar epithelial cells through sirtuin-1 signaling".JOURNAL OF INTERNATIONAL MEDICAL RESEARCH 49(2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
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