Functional Doxorubicin-Loaded Omega-3 Unsaturated Fatty Acids Nanoparticles in Reversing Hepatocellular Carcinoma Multidrug Resistance
文献类型：期刊论文
| 作者 | Wang, Chunlei1,2; Wei, Xiaoyan1,2; Shao, Guoliang1,2 |
| 刊名 | MEDICAL SCIENCE MONITOR
 |
| 出版日期 | 2021-02-01 |
| 卷号 | 27 |
| ISSN号 | 1643-3750 |
| 关键词 | Carcinoma, Hepatocellular Docosahexaenoic Acids Doxorubicin Nanoparticles Tuberculosis, Multidrug-Resistant |
| DOI | 10.12659/MSM.927727 |
| 通讯作者 | Shao, Guoliang(shaogl@zjcc.org.cn) |
| 英文摘要 | Background: This study investigated a nanoparticle drug delivery system to reverse multidrug resistance (MDR) and assessed its anticancer efficacy in hepatocellular carcinoma (HCC). Material/Methods: Docosahexaenoic acid (DHA) was used as the functional excipient and doxorubicin (DOX) as the chemotherapeutic drug to synthesize DOX nanoparticles (DOX-nano). The human HCC cell line HepG2 was used for experiments. HepG2/DOX, HepG2+DOX, HepG2+DOX-nano, HepG2/DOX+DOX, and HepG2/DOX+DOX-nano groups cells were treated with DOX or DOX-nano (5 mu g/mL). Nude mice bearing a HepG2/DOX xenograft were divided into model, DOX, vector-nano, and DOX-nano groups and injected with saline, DOX reagent, vector-nano, and DOX-nano (2 mg/kg), respectively. Next, cytotoxicity, cellular uptake, cell apoptosis and migration, fluorescence imaging, TUNEL assay, and tumor inhibition effects were assessed in vitro and in vivo. Furthermore, expression of MDR-related proteins was also detected using western blotting. Results: Fluorescence imaging showed that the DOX uptake in the DOX-nano-treated group was the strongest in the HCC cells or tumors. Cell apoptosis was significantly increased in DOX-nano-treated HepG2/DOX cells and tumors, and cell migration was significantly inhibited in the DOX-nano-treated HepG2/DOX cells compared with the other groups. The tumor inhibitory rate in DOX-nano-injected tumors was also significantly higher than in other groups. The expression of breast cancer resistance protein, B-cell lymphoma 2, lung resistance protein, multidrug resistance protein, and protein kinase C alpha was significantly decreased in DOX-nano-treated HepG2/DOX cells and xenograft tumors. Significantly better antitumor and MDR-reversing effects were also observed in the HepG2+DOX group compared with the HepG2/DOX group. Conclusions: This study revealed the potential efficacy of a DOX-nano drug delivery system for the treatment of HCC, using HepG2/DOX cells and nude mice bearing HepG2/DOX xenografts. |
| WOS关键词 | CO-DELIVERY ; CHEMOTHERAPY ; NANOCARRIERS ; TUMORS ; DRUGS ; CELLS ; DHA |
| 资助项目 | Medical Health Science and Technology Project of Zhejiang Provincial Health Commission, China[2018KY287] |
| WOS研究方向 | Research & Experimental Medicine |
| 语种 | 英语 |
| 出版者 | INT SCIENTIFIC INFORMATION, INC |
| WOS记录号 | WOS:000613544700001 |
| 资助机构 | Medical Health Science and Technology Project of Zhejiang Provincial Health Commission, China |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/119698]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Shao, Guoliang |
| 作者单位 | 1.Univ Chinese Acad Sci, Pharmaceut Preparat Sect, Canc Hosp, Zhejiang Canc Hosp, Hangzhou, Zhejiang, Peoples R China 2.Chinese Acad Sci, Inst Canc & Basic Med IBMC, Hangzhou, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Chunlei,Wei, Xiaoyan,Shao, Guoliang. Functional Doxorubicin-Loaded Omega-3 Unsaturated Fatty Acids Nanoparticles in Reversing Hepatocellular Carcinoma Multidrug Resistance[J]. MEDICAL SCIENCE MONITOR,2021,27. |
| APA | Wang, Chunlei,Wei, Xiaoyan,&Shao, Guoliang.(2021).Functional Doxorubicin-Loaded Omega-3 Unsaturated Fatty Acids Nanoparticles in Reversing Hepatocellular Carcinoma Multidrug Resistance.MEDICAL SCIENCE MONITOR,27. |
| MLA | Wang, Chunlei,et al."Functional Doxorubicin-Loaded Omega-3 Unsaturated Fatty Acids Nanoparticles in Reversing Hepatocellular Carcinoma Multidrug Resistance".MEDICAL SCIENCE MONITOR 27(2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
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