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A study of hydrophobins-modified menaquinone-7 on osteoblastic cells differentiation

文献类型：期刊论文

作者Tang, Hengfang1,2; Zhu, Zhu1,2; Zheng, Zhiming1,3; Wang, Han1; Li, Chu1; Wang, Li1; Zhao, Genhai1; Wang, Peng1,3
刊名MOLECULAR AND CELLULAR BIOCHEMISTRY
出版日期2021-01-27
关键词Menaquinone-7 Hydrophobins Alkaline phosphatase Osteocalcin OPG RANKL Mitochondria
ISSN号0300-8177
DOI10.1007/s11010-021-04062-z
通讯作者Zheng, Zhiming(zhengzhiming2014@163.com) ; Wang, Peng(pengwang@ipp.ac.cn)
英文摘要Menaquinone-7 is involved in bone metabolism and can be used to prevent and treat osteoporosis. However, as a fat-soluble vitamin, menaquinone-7 has poor water solubility. As a surfactant, hydrophobins can change the affinity/hydrophobicity of the covered interface. In this study, menaquinone-7 was modified by hydrophobins, and the different addition ratios were explored. Moreover, Fourier transform infrared (FTIR), X-ray photoelectron spectroscopy (XPS), and water contact angle (WCA) measurements indicated that hydrophobins effectively bind to menaquinone-7 and greatly increase the hydrophilicity of the surface of menaquinone-7. Studies on the metabolism of MC3T3-E1 cells showed that compared with native menaquinone-7, HGFI-modified menaquinone-7 can significantly promote osteoblast differentiation but inhibit osteoclast differentiation. Besides, the Mito-Tracker Green experiments show that HGFI-modified menaquinone-7 can significantly promote the activity of mitochondria in cells. These findings indicate that hydrophobins can be used as an effective biomaterial to modify menaquinone-7, promote the formation of osteoblasts, and better to bone balance.
资助项目National Key R&D Program of China[2019YFA0904300] ; National Key R&D Program of China[2019YFA0904304] ; Key research and development plan of Anhui Province[1804b06020342] ; Natural Science Foundation of Anhui Province[1908085MB48] ; Natural Science Foundation of Anhui Province[1908085MB43]
WOS研究方向Cell Biology
语种英语
WOS记录号WOS:000612261100001
出版者SPRINGER
资助机构National Key R&D Program of China ; Key research and development plan of Anhui Province ; Natural Science Foundation of Anhui Province
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/119784]  
专题中国科学院合肥物质科学研究院
通讯作者Zheng, Zhiming; Wang, Peng
作者单位1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Peoples R China
2.Univ Sci & Technol China, Sci Isl Branch Grad, Hefei 230026, Peoples R China
3.Chinese Acad Sci, Hefei Inst Technol Innovat Engn, Hefei, Peoples R China
推荐引用方式
GB/T 7714		 Tang, Hengfang,Zhu, Zhu,Zheng, Zhiming,et al. A study of hydrophobins-modified menaquinone-7 on osteoblastic cells differentiation[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY,2021.
APA Tang, Hengfang.,Zhu, Zhu.,Zheng, Zhiming.,Wang, Han.,Li, Chu.,...&Wang, Peng.(2021).A study of hydrophobins-modified menaquinone-7 on osteoblastic cells differentiation.MOLECULAR AND CELLULAR BIOCHEMISTRY.
MLA Tang, Hengfang,et al."A study of hydrophobins-modified menaquinone-7 on osteoblastic cells differentiation".MOLECULAR AND CELLULAR BIOCHEMISTRY (2021).

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来源：合肥物质科学研究院

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