中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 合肥物质科学研究院
  3. 中国科学院合肥物质科学研究院
Secondary structure and transmembrane topology analysis of the N-terminal domain of the inner membrane protein EccE(1) from M. smegmatis using site-directed spin labeling EPR

文献类型：期刊论文

作者Yu, Lu1; Fang, Wei2,3; He, Yao2,3; Cai, Wenguang4; Wei, Wei5; Tian, Changlin1,2,3
刊名BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
出版日期2021-02-01
卷号1863
ISSN号0005-2736
关键词Mycobacterial ESX-1 secretion system Membrane protein EccE(1) Site-directed spin labeling Electron Paramagnetic Resonance Secondary structure Transmembrane topology
DOI10.1016/j.bbamem.2020.183515
通讯作者Yu, Lu(luyu@hmfl.ac.cn) ; Tian, Changlin(cltian@ustc.edu.cn)
英文摘要Protein EccE(1) is an essential component of the mycobacterial ESX-1 secretion system, which plays a crucial part in the process of virulence factors secretion, especially for pathogenic mycobacteria such as Mycobacterium tuberculosis. While EccE(1) was previously postulated to be the inner membrane pore-forming unit of a membrane complex through which substrates are transported, the structural properties of EccE(1) remains to be explored. In the present study, systematic Site-Directed Spin Labeling (SDSL) and Electron Paramagnetic Resonance (EPR) spectroscopic studies was carried out to reveal the secondary structure and transmembrane topology of the Nterminal Domain of EccE(1) protein (EccE(1)-NTD) from M. smegmatis in detergent micelles. EPR-based mobility and accessibility analysis of the R1 side chain for 64 residue positions of EccE(1) -NTD indicates that the transmembrane domain adopts two a-helices spanning Phe7-Cys30 and Leu36-Ile54. A tentative structural topology model of EccE(1) -NTD embedded in membrane is also suggested based on EPR spectroscopic data in this study, which will provide further insights into this protein and the ESX secretion systems of mycobacteria.
WOS关键词VII SECRETION ; ESX-1
资助项目Natural Science Foundation of China[21825703] ; Natural Science Foundation of China[U1732275] ; Natural Science Foundation of China[21927814] ; Natural Science Foundation of China[U1832181] ; Natural Science Foundation of China[31500611] ; National Key Research and Development Program of China[2019YFA0405600] ; Users with Excellence Program of Hefei Science Center, CAS[2018HSC-UE013] ; Users with Excellence Program of Hefei Science Center, CAS[2019HSC-UE005] ; Users with Excellence Program of Hefei Science Center, CAS[2019HSC-UE020] ; Major Program of Development Foundation of Hefei Center for Physical Science and Technology[2018ZYFX003]
WOS研究方向Biochemistry & Molecular Biology ; Biophysics
语种英语
出版者ELSEVIER
WOS记录号WOS:000603419900018
资助机构Natural Science Foundation of China ; National Key Research and Development Program of China ; Users with Excellence Program of Hefei Science Center, CAS ; Major Program of Development Foundation of Hefei Center for Physical Science and Technology
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/119956]  
专题中国科学院合肥物质科学研究院
通讯作者Yu, Lu; Tian, Changlin
作者单位1.Chinese Acad Sci, High Magnet Field Lab, Hefei 230031, Anhui, Peoples R China
2.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230026, Anhui, Peoples R China
3.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China
4.Anhui Univ, Sch Life Sci, 111 Jiulong Rd, Hefei 230601, Anhui, Peoples R China
5.Univ Sci & Technol China, Dept Chem, Hefei 230026, Peoples R China
推荐引用方式
GB/T 7714		 Yu, Lu,Fang, Wei,He, Yao,et al. Secondary structure and transmembrane topology analysis of the N-terminal domain of the inner membrane protein EccE(1) from M. smegmatis using site-directed spin labeling EPR[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES,2021,1863.
APA Yu, Lu,Fang, Wei,He, Yao,Cai, Wenguang,Wei, Wei,&Tian, Changlin.(2021).Secondary structure and transmembrane topology analysis of the N-terminal domain of the inner membrane protein EccE(1) from M. smegmatis using site-directed spin labeling EPR.BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES,1863.
MLA Yu, Lu,et al."Secondary structure and transmembrane topology analysis of the N-terminal domain of the inner membrane protein EccE(1) from M. smegmatis using site-directed spin labeling EPR".BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 1863(2021).

入库方式： OAI收割

来源：合肥物质科学研究院

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。