A flexible and cost-effective manual droplet operation platform for miniaturized cell assays and single cell analysis
文献类型：期刊论文
| 作者 | Lou, Qi1,2,3,4; Ma, Yan1,2,3,4; Zhao, Shi-Ping1,5; Du, Guan-Sheng1; Fang, Qun1 |
| 刊名 | TALANTA
 |
| 出版日期 | 2021-03-01 |
| 卷号 | 224 |
| 关键词 | Manual droplet operation system (MDOS) Nanoliter to picoliter range Miniaturized cell assays Drug test Single cell assay |
| ISSN号 | 0039-9140 |
| 通讯作者 | Fang, Qun(fangqun@zju.edu.cn) |
| 英文摘要 | Herein, we developed a flexible and cost-effective manual droplet operation system (MDOS) for performing miniaturized cell assays as well as single cell analysis. The MDOS consists of a manual x-y-z translation stage for liquid transferring and switching, a high-precision syringe pump for liquid driving and metering, a tapered capillary probe for droplet manipulation, a droplet array chip for droplet loading and reaction, sample/reagent reservoirs for storage, and a microscope for droplet observation, with a total expense of only $4,000. By using the flexible combination of three elementary operations of the x-y-z stage's moving and the pump's aspirating and depositing, the MDOS can manually achieve multiple droplet handling operations in the nanoliter to picoliter range, including droplet generation, assembling, fusion, diluting, and splitting. On this basis, multiple cell-related operations could be performed, such as nanoliter-scale in-droplet cell culture, cell coculture, drug stimulation, cell washing, and cell staining, as well as formation of picoliter single-cell droplets. The feasibility and flexibility of the MDOS was demonstrated in multi-mode miniaturized cell assays, including cell-based drug test, first-pass effect assay, and single-cell enzyme assay. The MDOS with the features of low cost, easy to build and flexible to use, could provide a promising alternative for performing miniaturized assays in routine laboratories, in addition to conventional microfluidic chip-based systems and automated robot systems. |
| WOS关键词 | MICROFLUIDIC PLATFORM ; MASS-SPECTROMETRY ; THROUGHPUT ; SYSTEM ; ARRAY ; CHIP ; CULTURE ; ENCAPSULATION ; ABSORPTION ; MIGRATION |
| 资助项目 | Natural Science Foundation of China[21827806] ; Natural Science Foundation of China[21435004] ; Natural Science Foundation of China[21227007] ; Fundamental Research Funds for the Zhejiang Province Universities[2019XZZX003-15] ; Fundamental Research Project of the Central Universities[2-2050205-19-007] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000600787800087 |
| 出版者 | ELSEVIER |
| 资助机构 | Natural Science Foundation of China ; Fundamental Research Funds for the Zhejiang Province Universities ; Fundamental Research Project of the Central Universities |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/120065]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Fang, Qun |
| 作者单位 | 1.Zhejiang Univ, Inst Microanalyt Syst, Dept Chem, Key Lab Biomed Engn,Minist Educ, Hangzhou 310058, Peoples R China 2.Chinese Acad Sci, Inst Canc & Basic Med ICBM, Hangzhou 310022, Peoples R China 3.Univ Chinese Acad Sci, Canc Hosp, Hangzhou 310022, Peoples R China 4.Zhejiang Canc Hosp, Hangzhou 310022, Peoples R China 5.Shaoxing Ecol Environm Bur, Shangyu Branch, Shaoxing 312300, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lou, Qi,Ma, Yan,Zhao, Shi-Ping,et al. A flexible and cost-effective manual droplet operation platform for miniaturized cell assays and single cell analysis[J]. TALANTA,2021,224. |
| APA | Lou, Qi,Ma, Yan,Zhao, Shi-Ping,Du, Guan-Sheng,&Fang, Qun.(2021).A flexible and cost-effective manual droplet operation platform for miniaturized cell assays and single cell analysis.TALANTA,224. |
| MLA | Lou, Qi,et al."A flexible and cost-effective manual droplet operation platform for miniaturized cell assays and single cell analysis".TALANTA 224(2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
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