Au@Ag core-shell nanoparticles for microRNA-21 determination based on duplex-specific nuclease signal amplification and surface-enhanced Raman scattering
文献类型：期刊论文
| 作者 | Xu, Wei1,2,3 ; Zhao, Aiwu1,2,3; Zuo, Fangtao1,2,3; Khan, Ranjha4; Hussain, Hafiz Muhammad Jafar4; Chang, Jianguo1,2
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| 刊名 | MICROCHIMICA ACTA
 |
| 出版日期 | 2020-06-12 |
| 卷号 | 187 |
| 关键词 | Au@DTNB@Ag core-shell nanoparticles Surface-enhanced Raman scattering miRNA-21 Duplex-specific nuclease Human serum |
| ISSN号 | 0026-3672 |
| DOI | 10.1007/s00604-020-04330-7 |
| 通讯作者 | Zhao, Aiwu(awzhao@iim.ac.cn) |
| 英文摘要 | A novel surface-enhanced Raman scattering (SERS) analysis strategy has been designed combining Au@DTNB@Ag core-shell nanoparticles (DTNB attachment on gold nanoparticles, then encapsulated in Ag shell nanoparticles named as ADANPs) and duplex-specific nuclease signal amplification (DSNSA) platform. Firstly, ADANPs and magnetic substrate of Fe3O4 nanoparticles were covalently attached to the 3 '- and 5 '- end of capture probe (CP) targeting miRNA-21. Upon the addition of target miRNA-21, these heteroduplexes were specifically cleaved by DSN and resulted in ADANPs that were released from the surface of Fe3O4 nanoparticles (Fe3O4 NPs). At the same time, miRNA-21 remained intact and can rehybridize another DNA probe to trigger the signal-amplifying reaction. Based on this principle, the developed SERS method exhibited good linearity in the range 0 to 1 nM for miRNA-21 with a limit of detection (LOD) of 0.084 fM and has an ability to differentiate even a single-base mismatched sequence on the target sequence or other miRNA sequence. The results provide a novel SERS method which can successfully been applied to the miRNA-21 detection in human serum. |
| WOS关键词 | QUANTITATIVE DETECTION ; SPECTROSCOPY PLATFORM ; ONE-STEP ; BIOSENSOR ; STRATEGY ; GO |
| 资助项目 | National Natural Science Foundation of China[61875255] ; Hefei Center of Physical Science Technology[2018ZYFX005] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000542241300002 |
| 出版者 | SPRINGER WIEN |
| 资助机构 | National Natural Science Foundation of China ; Hefei Center of Physical Science Technology |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/71409]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Zhao, Aiwu |
| 作者单位 | 1.Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Dept Chem, Hefei 230026, Anhui, Peoples R China 3.Chinese Acad Sci, State Key Lab Transducer Technol, Hefei 230031, Peoples R China 4.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Wei,Zhao, Aiwu,Zuo, Fangtao,et al. Au@Ag core-shell nanoparticles for microRNA-21 determination based on duplex-specific nuclease signal amplification and surface-enhanced Raman scattering[J]. MICROCHIMICA ACTA,2020,187. |
| APA | Xu, Wei,Zhao, Aiwu,Zuo, Fangtao,Khan, Ranjha,Hussain, Hafiz Muhammad Jafar,&Chang, Jianguo.(2020).Au@Ag core-shell nanoparticles for microRNA-21 determination based on duplex-specific nuclease signal amplification and surface-enhanced Raman scattering.MICROCHIMICA ACTA,187. |
| MLA | Xu, Wei,et al."Au@Ag core-shell nanoparticles for microRNA-21 determination based on duplex-specific nuclease signal amplification and surface-enhanced Raman scattering".MICROCHIMICA ACTA 187(2020). |
入库方式： OAI收割
来源：合肥物质科学研究院
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