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A genetically encoded small-size fluorescent pair reveals allosteric conformational changes of G proteins upon its interaction with GPCRs by fluorescence lifetime based FRET

文献类型：期刊论文

作者Shi, Pan1,2; Zhang, Yanan1,2; Lv, Pei1,2; Fang, Wei1,2; Ling, Shenglong1,2; Guo, Xiaoqi1,2; Li, Dong1,2; Liu, Sanling1,2; Sun, Demeng1,2; Zhang, Longhua1,2
刊名CHEMICAL COMMUNICATIONS
出版日期2020-06-28
卷号56
ISSN号1359-7345
DOI10.1039/d0cc02691c
通讯作者Shi, Pan(shipan@ustc.edu.cn) ; Tian, Changlin(cltian@ustc.edu.cn)
英文摘要The dynamics of GPCRs (G protein-coupled receptors) coupling for cognate G proteins play a critical role in signal transduction. Herein, we reported a site-specifically labelled small-sized fluorescent pair 7-HC/FlAsH ((7-hydroxycoumarin-4-yl)-ethylglycine/fluorescein arsenical hairpin) for fluorescence lifetime based FRET (fluorescence resonance energy transfer) to reveal conformational differences of G alpha(i1)(inhibitory G proteins) and G alpha(s)(stimulatory G proteins) upon beta(2)AR (beta(2)-adrenergic receptor) coupling. It offers a new generally applicable method to probe protein dynamic interactions or conformational changes.
WOS关键词ALPHA-SUBUNIT ; ACTIVATION ; NUCLEOTIDE
资助项目National Key Research and Development Project of China[2016YFA0400903] ; National Key Research and Development Project of China[2017YFA0505201] ; National Key Research and Development Project of China[2017YFA0505400] ; National Natural Science Foundation of China[21825703] ; National Natural Science Foundation of China[31971152]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000542898200002
出版者ROYAL SOC CHEMISTRY
资助机构National Key Research and Development Project of China ; National Natural Science Foundation of China
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/71362]  
专题中国科学院合肥物质科学研究院
通讯作者Shi, Pan; Tian, Changlin
作者单位1.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Peoples R China
2.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Peoples R China
3.ShanghaiTech Univ, iHuman Inst, 99 HaiKe Rd, Shanghai 201210, Peoples R China
4.Chinese Acad Sci, High Magnet Field Lab, Hefei 230030, Peoples R China
5.Univ Sci & Technol China, Anhui Lab Adv Photon Sci & Technol, Hefei 230026, Peoples R China
推荐引用方式
GB/T 7714		 Shi, Pan,Zhang, Yanan,Lv, Pei,et al. A genetically encoded small-size fluorescent pair reveals allosteric conformational changes of G proteins upon its interaction with GPCRs by fluorescence lifetime based FRET[J]. CHEMICAL COMMUNICATIONS,2020,56.
APA Shi, Pan.,Zhang, Yanan.,Lv, Pei.,Fang, Wei.,Ling, Shenglong.,...&Tian, Changlin.(2020).A genetically encoded small-size fluorescent pair reveals allosteric conformational changes of G proteins upon its interaction with GPCRs by fluorescence lifetime based FRET.CHEMICAL COMMUNICATIONS,56.
MLA Shi, Pan,et al."A genetically encoded small-size fluorescent pair reveals allosteric conformational changes of G proteins upon its interaction with GPCRs by fluorescence lifetime based FRET".CHEMICAL COMMUNICATIONS 56(2020).

入库方式： OAI收割

来源：合肥物质科学研究院

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