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Correlation Between Single Nucleotide Polymorphisms of an miRNA Binding Site in the 3 ' UTR ofPTENand Risk of Cervical Cancer Among the Han Chinese

文献类型：期刊论文

作者Yu, Aijun1,2; Zhang, Jiejie1,2; Mei, Yuxian3; Zhong, Hai4; Chen, Shasha5; Song, Qian6
刊名GENETIC TESTING AND MOLECULAR BIOMARKERS
出版日期2020-07-01
卷号24
ISSN号1945-0265
关键词cervical cancer PTEN single nucleotide polymorphism miRNA
DOI10.1089/gtmb.2019.0269
通讯作者Chen, Shasha(CSSCSS724724@163.com) ; Song, Qian(happysongqian@126.com)
英文摘要Objective:To analyze the association between a single nucleotide polymorphism (SNP) of the 3 ' untranslated region (UTR) of the phosphatase and tensin homolog (PTEN)gene, that is within a microRNA (miRNA) binding site, and the risk of Chinese Han cervical cancer. Methods:A case-control study was carried out to analyze the genotype of thePTENrs34140758 locus in 210 surgically treated, Han Chinese, cervical cancer patients and 210 healthy controls. The levels of the miRNAs hsa-miR-586 and hsa-miR-622 and thePTENmRNA were analyzed by real-time reverse transcription-quantitative polymerase chain reaction in the cancerous and adjacent normal tissues from all cases. HeLa cells were transfected with the miRNAs, hsa-miR-586 and hsa-miR-622, to analyze their effects on PTEN gene expression. Results:After adjusting for age, body-mass index, alcohol consumption, smoking, and familial history of cancer, thePTENrs34140758 A allele carriers were 1.47 times more likely to suffer from cervical cancer than the C allele carriers (odds ratio [OR] = 1.47, 95% confidence interval [CI]: 1.17-1.72,p = 0.001). Both hsa-miR-586 and hsa-miR-622 were highly expressed in the cancerous tissues of the cervical cancer patients, whereasPTENexpression was low. HeLa cell transfection experiments showed that hsa-miR-586 and hsa-miR-622 inhibitedPTENgene expression. The results of a dual-luciferase reporter assay showed that thePTENgene is a target for both hsa-miR-586 and hsa-miR-622. Conclusion:ThePTEN3 ' UTR rs34140758 locus SNP is associated with the risk of cervical cancer in the Han Chinese population. The molecular mechanism may be that the rs34140758 SNP affects the regulation ofPTENgene expression through interaction with the hsa-miR-586 and hsa-miR-622 miRNAs.
WOS关键词PTEN ; EXPRESSION ; MIGRATION ; DIAGNOSIS ; INVASION ; MIR-622
资助项目Zhejiang Provincial Medicine Health Science and Technology Program[2019ZA021]
WOS研究方向Biochemistry & Molecular Biology ; Genetics & Heredity
语种英语
出版者MARY ANN LIEBERT, INC
WOS记录号WOS:000541879100001
资助机构Zhejiang Provincial Medicine Health Science and Technology Program
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/71350]  
专题中国科学院合肥物质科学研究院
通讯作者Chen, Shasha; Song, Qian
作者单位1.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp, Dept Gynecol Oncol, Hangzhou, Peoples R China
2.Chinese Acad Sci, Inst Canc & Basic Med IBMC, Hangzhou, Peoples R China
3.Wenling Hosp Tradit Chinese Med, Dept Urol, Wenling, Zhejiang, Peoples R China
4.Second Hosp Yinzhou Dist, Dept Cardiothorac Surg, Ningbo, Zhejiang, Peoples R China
5.Taizhou Canc Hosp, Dept Tradit Chinese Med, 50 Zhenxin Rd, Wenling 317502, Zhejiang, Peoples R China
6.Taizhou Canc Hosp, Dept Gynecol & Obstet, 50 Zhenxin Rd, Wenling 317502, Zhejiang, Peoples R China
推荐引用方式
GB/T 7714		 Yu, Aijun,Zhang, Jiejie,Mei, Yuxian,et al. Correlation Between Single Nucleotide Polymorphisms of an miRNA Binding Site in the 3 ' UTR ofPTENand Risk of Cervical Cancer Among the Han Chinese[J]. GENETIC TESTING AND MOLECULAR BIOMARKERS,2020,24.
APA Yu, Aijun,Zhang, Jiejie,Mei, Yuxian,Zhong, Hai,Chen, Shasha,&Song, Qian.(2020).Correlation Between Single Nucleotide Polymorphisms of an miRNA Binding Site in the 3 ' UTR ofPTENand Risk of Cervical Cancer Among the Han Chinese.GENETIC TESTING AND MOLECULAR BIOMARKERS,24.
MLA Yu, Aijun,et al."Correlation Between Single Nucleotide Polymorphisms of an miRNA Binding Site in the 3 ' UTR ofPTENand Risk of Cervical Cancer Among the Han Chinese".GENETIC TESTING AND MOLECULAR BIOMARKERS 24(2020).

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来源：合肥物质科学研究院

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