Cdk phosphorylation licenses Kif4A chromosome localization required for early mitotic progression
文献类型：期刊论文
| 刊名 | JOURNAL OF MOLECULAR CELL BIOLOGY
 |
| 出版日期 | 2018 |
| 卷号 | 10 |
| 关键词 | ANAPHASE CENTRAL SPINDLE CHROMOKINESIN KIF4A MIDZONE FORMATION EJECTION FORCES SMC COMPLEXES CYTOKINESIS PROTEIN PRC1 CONGRESSION CYCLE Kif4A chromokinesin Cdk phosphorylation mitosis |
| ISSN号 | 1674-2788 |
| 其他题名 | Cdk phosphorylation licenses Kif4A chromosome localization required for early mitotic progression |
| 英文摘要 | The chromokinesin Kif4A controls proper chromosome condensation, congression/alignment, and cytokinesis to ensure faithful genetic inheritance. Here, we report that Cdk phosphorylation of human Kif4A at T1161 licenses Kif4A chromosomal localization, which, in turn, controls Kif4A early mitotic function. Phosphorylated Kif4A (Kif4A(WT)) or Cdk phospho-mimetic Kif4A mutant (Kif4A(TE)) associated with chromosomes and condensin I (non-SMC subunit CAP-G and core subunit SMC2) to regulate chromosome condensation, spindle morphology, and chromosome congression/alignment in early mitosis. In contrast, Cdk nonphosphorylatable Kif4A mutant (Kif4A(TA)) could neither localize on chromosomes nor associate with CAP-G and SMC2. Furthermore, Kif4A(TA) could not rescue defective chromosome condensation, spindle morphology, or chromosome congression/alignment in cells depleted of endogenous Kif4A, which activated a mitotic checkpoint and delayed early mitotic progression. However, targeting Kif4A(TA) to chromosomes by fusion of Kif4A(TA) with Histone H1 resulted in restoration of chromosome and spindle functions of Kif4A, similar to Kif4A(WT) and Kif4A(TE), in cells depleted of endogenous Kif4A. Thus, our results demonstrate that Cdk phosphorylationlicensed chromosomal localization of Kif4A plays a critical role in regulating early mitotic functions of Kif4A that are important for early mitotic progression. |
| 资助项目 | [CAMS Innovation Fund for Medical Sciences (CIFMS)] ; [National Natural Science Foundation of China] ; [National Basic Research Program of China] ; [Tianjin Research Program of Application Foundation and Advanced Technology] ; [Program for New Century Excellent Talents in University in China] |
| 语种 | 英语 |
| CSCD记录号 | CSCD:6347813 |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/65735]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 推荐引用方式 GB/T 7714 | . Cdk phosphorylation licenses Kif4A chromosome localization required for early mitotic progression[J]. JOURNAL OF MOLECULAR CELL BIOLOGY,2018,10. |
| APA | (2018).Cdk phosphorylation licenses Kif4A chromosome localization required for early mitotic progression.JOURNAL OF MOLECULAR CELL BIOLOGY,10. |
| MLA | "Cdk phosphorylation licenses Kif4A chromosome localization required for early mitotic progression".JOURNAL OF MOLECULAR CELL BIOLOGY 10(2018). |
入库方式： OAI收割
来源：合肥物质科学研究院
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