The study of absorption spectrum for cell substrate
文献类型：期刊论文
| 刊名 | SPECTROSCOPY AND SPECTRAL ANALYSIS
 |
| 出版日期 | 2004 |
| 卷号 | 24 |
| 关键词 | RAMAN-SPECTROSCOPY BLOOD cell culture substrate absorption spectrum |
| ISSN号 | 1000-0593 |
| 其他题名 | The Study of Absorption Spectrum for Cell Substrate |
| 英文摘要 | The authors collected the absorption spectrum of RPMI 1640 and DMEM substrates that cultivated Hela and CNE by UV-3101 spectrophotometer and analysed the absorbability of proteins in the substrate. The absorption peaks of the RPMI 1 640 culture medium that cultivated cells for different times shifted from 227 to 222 or 218 nm and from 278 to 280 nm respectively; while during growing course of cultivated cells, one of the absorption peaks of DMEM culture medium shifted from 224 nm to one near 221 nm, and the absorption peak 278 nm almost had no shift. All of these shifts show that the content of each amino acid such as tryptophan and casein has already changed. That is, during the growing course of cultivating cancer cells, the tryptophan and casein were not depleted equivalently. In the growth period of Hela and CNE, they consumed different amino acid. So they need different component proportion for amino acid. |
| 语种 | 英语 |
| CSCD记录号 | CSCD:1791649 |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/64691]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 推荐引用方式 GB/T 7714 | . The study of absorption spectrum for cell substrate[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2004,24. |
| APA | (2004).The study of absorption spectrum for cell substrate.SPECTROSCOPY AND SPECTRAL ANALYSIS,24. |
| MLA | "The study of absorption spectrum for cell substrate".SPECTROSCOPY AND SPECTRAL ANALYSIS 24(2004). |
入库方式： OAI收割
来源：合肥物质科学研究院
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