The expression of AQP(1-10) mRNA in the human mesothelial cells
文献类型：期刊论文
| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2007 |
| 卷号 | 34 |
| 关键词 | WATER CHANNELS AQUAPORINS human mesothelial cell culture aquaporins |
| ISSN号 | 1000-3282 |
| 其他题名 | The Expression of AQP_(1-10) mRNA in The Human Mesothelial Cells |
| 英文摘要 | Aquaporins (AQP) are integral membrane proteins that serve as channels in the transfer of water, and in some cases, small solutes across the membrane. In order to detect the expression of AQP(1 similar to 10) mRNA in the human pleural mesothelial cells, culture reproducible model of human pleural mesothelial cells was established in vitro. The value of aquaporin water channels was investigated in pleural fluid dynamics. Mesothelial cells were isolated from pleural effusion fluid of nonmalignant pleural effusion patients and cultured in vitro. The mesothelial cells were identified by morphology and streptomyces protein-peroxidase(S-P) procedure. RT-PCR was used to detect the expression of AQP(1 similar to 10) mRNA in human pleural mesothelial cells. Establishment of culture reproducible model achieved success of human pleural mesothelial cells in vitro. Immunocytochemistry revealed that cytokeratin and vimentin expressed positive, V III factor associated antigen and CD45 was negtive. Confluent human pleural mesothelial cells appeared multipolar and like cabblestone. Under electron microscopy numerous surface microvilli and abundant endoplasmic reticulum were observed. The cells was identified that it was mesothelial cells. The cells isolated from human pleural effusion fluid have higher purity quotient. RT-PCR studies showed that all of the AQP(1 similar to 10) mRNA expressed in mesothelial cells. AQP(1), AQP(9), AQP(10) mRNA have abundant expression in mesothelial cells and significant deviation was found between AQP(1), AQP(9), AQP(10) and AQP(2), AQP(3), AQP(7), AQP(8). Human pleural mesothelial cells expressed AQP(1 similar to 10) mRNA, all of them have relation with the function of human pleural mesothelial cells. It was confirmed that mesothelial cells have important contribution in pleural fluid dynamics. Aquaporins (AQP) are involved in rapid and active gating of water across biological membranes. Expression of aquaporins was intervened, new treat method may be found of pleural effusion. |
| 语种 | 英语 |
| CSCD记录号 | CSCD:2736703 |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/60114]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 推荐引用方式 GB/T 7714 | . The expression of AQP(1-10) mRNA in the human mesothelial cells[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2007,34. |
| APA | (2007).The expression of AQP(1-10) mRNA in the human mesothelial cells.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,34. |
| MLA | "The expression of AQP(1-10) mRNA in the human mesothelial cells".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 34(2007). |
入库方式： OAI收割
来源：合肥物质科学研究院
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