Knockdown of c-Myc inhibits cell proliferation by negatively regulating the Cdk/Rb/E2F pathway in nasopharyngeal carcinoma cells
文献类型：期刊论文
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2015 |
| 卷号 | 47 |
| 关键词 | HEPATOCELLULAR-CARCINOMA TARGET GENE IN-VIVO EXPRESSION INACTIVATION ONCOGENE GROWTH CANCER CYCLE TUMORIGENESIS nasopharyngeal carcinoma c-Myc 5-8F cell growth Cdk/Rb/E2F pathway |
| ISSN号 | 1672-9145 |
| 其他题名 | Knockdown of c-Myc inhibits cell proliferation by negatively regulating the Cdk/Rb/E2F pathway in nasopharyngeal carcinoma cells |
| 英文摘要 | The proto-oncogene c-Myc encodes a transcription factor that is involved in the regulation of cellular proliferation, differentiation, and apoptosis. Several studies indicate that the over-expression of c-Myc is a frequent genetic abnormality in nasopharyngeal carcinoma (NPC). Therefore, specifically reducing its level by genetic means in established NPC cell lines helps to better understand its role in the pathogenesis of NPC. In this study, for the first time, we successfully established and characterized NPC 5-8F cell line with stably suppressed c-Myc expression by employing a DNA-based RNA interference approach. The suppression of c-Myc resulted in reduced cell growth, colony formation, and cell cycle progression in 5-8F cells. In vivo tumor formation assays revealed that the knockdown of c-Myc reduced the tumorigenic potential of 5-8F cells in nude mice. At the molecular level, we found that the knockdown of c-Myc could decrease the expression of several critical molecules involved in the Cdk/Rb/E2F pathway, including CDK4, cyclin D1, CDK2, pRb, E2F3, and DP2, and significantly reduced the promoter activity of cyclin D1. Taken together, these findings provide valuable mechanistic insights into the role of c-Myc in nasopharyngeal carcinogenesis and suggest that the knockdown of c-Myc may be a potential therapeutic approach for the treatment of NPC. |
| 资助项目 | [National Natural Science Foundation of China] ; [Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry (the 44th)] |
| 语种 | 英语 |
| CSCD记录号 | CSCD:5382526 |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/58661]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 推荐引用方式 GB/T 7714 | . Knockdown of c-Myc inhibits cell proliferation by negatively regulating the Cdk/Rb/E2F pathway in nasopharyngeal carcinoma cells[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2015,47. |
| APA | (2015).Knockdown of c-Myc inhibits cell proliferation by negatively regulating the Cdk/Rb/E2F pathway in nasopharyngeal carcinoma cells.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,47. |
| MLA | "Knockdown of c-Myc inhibits cell proliferation by negatively regulating the Cdk/Rb/E2F pathway in nasopharyngeal carcinoma cells".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 47(2015). |
入库方式： OAI收割
来源：合肥物质科学研究院
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。