Early urinary candidate biomarker discovery in a rat thioacetamide-induced liver fibrosis model
文献类型：期刊论文
| 刊名 | SCIENCE CHINA-LIFE SCIENCES
 |
| 出版日期 | 2018 |
| 卷号 | 61 |
| 关键词 | CARBONIC-ANHYDRASE-III HEPATOCELLULAR-CARCINOMA PROTEOMIC ANALYSIS HEPATIC-FIBROSIS CYSTATIN-C CIRRHOSIS SERUM EXPRESSION PROTEIN MARKER urine proteomics biomarker animal model liver fibrosis |
| ISSN号 | 1674-7305 |
| 其他题名 | Early urinary candidate biomarker discovery in a rat thioacetamide-induced liver fibrosis model |
| 英文摘要 | Biomarker is the change associated with the disease. Blood is relatively stable because of the homeostatic mechanisms of the body. However, urine accumulates changes of the body, which makes it a better early biomarker source. Liver fibrosis is a reversible pathological condition, whereas cirrhosis, the end-stage of liver fibrosis, is irreversible. Consequently, noninvasive early biomarkers for fibrosis are desperately needed. In this study, differential urinary proteins were identified in the thioacetamide liver fibrosis rat model using tandem mass tagging and two-dimensional liquid chromatography tandem mass spectrometry. A total of 766 urinary proteins were identified, 143 and 118 of which were significantly changed in the TAA 1-week and 3-week groups, respectively. Multiple reaction monitoring (MRM)-targeted proteomics was used to further validate the abundant differentially expressed proteins. A total of 40 urinary proteins were statistically significant, 15 of which had been previously reported as biomarkers of liver fibrosis, cirrhosis or other related diseases and 10 of which had been reported to be associated with the pathology and mechanism of liver fibrosis. These differential proteins were detected in urine before the alanine aminotransferase and aspartate transaminase changes in the serum and before fibrosis was observed upon hematoxylin and eosin (HE) and Masson's staining. |
| 资助项目 | [National Key Research and Development Program of China] ; [National Basic Research Program of China] ; [Beijing Natural Science Foundation] ; [Beijing Normal University] |
| 语种 | 英语 |
| CSCD记录号 | CSCD:6408927 |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/58250]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 推荐引用方式 GB/T 7714 | . Early urinary candidate biomarker discovery in a rat thioacetamide-induced liver fibrosis model[J]. SCIENCE CHINA-LIFE SCIENCES,2018,61. |
| APA | (2018).Early urinary candidate biomarker discovery in a rat thioacetamide-induced liver fibrosis model.SCIENCE CHINA-LIFE SCIENCES,61. |
| MLA | "Early urinary candidate biomarker discovery in a rat thioacetamide-induced liver fibrosis model".SCIENCE CHINA-LIFE SCIENCES 61(2018). |
入库方式： OAI收割
来源：合肥物质科学研究院
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