Determination of Cholesterol in Human Serum Based on Flow-injection Chemiluminescence Method by Sol-gel Immobilized Enzyme
文献类型：期刊论文
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2010 |
| 卷号 | 38 |
| 关键词 | ASSAY Sol-gel Immobilized enzyme column Flow injection Chemiluminescence Cholesterol Hemoglobin |
| ISSN号 | 0253-3820 |
| 其他题名 | Determination of Cholesterol in Human Serum Based on Flow-injection Chemiluminescence Method by Sol-gel Immobilized Enzyme |
| 英文摘要 | The cholesterol esterase and its oxidase were respectively immobilized onto sol-gel (tetraethyl orthosilicate) and then an enzymatic reaction column was prepared. Hydrolysis of cholesterol ester took place in the presence of cholesterol esterase to form cholesterol under catalysis of cholesterol oxidase, and Cholesterol was oxidized to produce hydrogen peroxide which reacted with luminal in the presence of simulated enzyme hemoglobin to result in the emission of light. Thereupon, a chemiluminescence system combined with flow injection technology by sol-gel immobilized enzyme was developed for the determination of cholesterol including free and total amount in human serum. The CL intensity was well linear with the concentration of cholesterol; liner range of total and free cholesterol was 1.01 x 10(-6) similar to 2.02 x 10(-4) mol/L with a detection limit of 7.5 x 10(-7) mol/L and 5.0 x 10(-8) similar to 2.18 x 10(-5) mol/L, with a detection limit of 5.0 x 10(-9) mol/L respectively. Contrasted the method with the biochemical analyzer (TBA-120FR), both the methods had no. significant difference. This method has been successfully applied for the detection of cholesterol in clinic serum samples. |
| 语种 | 英语 |
| CSCD记录号 | CSCD:3816046 |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/57040]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 推荐引用方式 GB/T 7714 | . Determination of Cholesterol in Human Serum Based on Flow-injection Chemiluminescence Method by Sol-gel Immobilized Enzyme[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2010,38. |
| APA | (2010).Determination of Cholesterol in Human Serum Based on Flow-injection Chemiluminescence Method by Sol-gel Immobilized Enzyme.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,38. |
| MLA | "Determination of Cholesterol in Human Serum Based on Flow-injection Chemiluminescence Method by Sol-gel Immobilized Enzyme".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 38(2010). |
入库方式： OAI收割
来源：合肥物质科学研究院
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