Reversal effects of droloxifene on multidrug resistance in adriamycin-resistant K562 cell line
文献类型：期刊论文
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2001 |
| 卷号 | 22 |
| 关键词 | K562 cells droloxifene multiple drug resistance |
| ISSN号 | 0253-9756 |
| 其他题名 | Reversal effects of droloxifene on multidrug resistance in adriamycin-resistant K562 cell line |
| 英文摘要 | AIM: To study the reversal effects of droloxifene (DRO) on multidrug resistance (MDR) in K562 cell line resistant to adriamycin (ADR). METHODS: K562 cell line resistant to ADR (K562/A02) and K562 cell line sensitive to ADR (K562) were treated with DRO. Using NM assay, chemosensitivity to ADR in DRO-treated K562 cell lines was studied. Before and after the treatment with DRO 10 mu mol/L, MDR1 and GST pi gene expression were assayed by reverse transcription-polymerase chain reaction and immunocytochemistry assay. Flow cytometry was used to determine intracellular ADR concentration. RESULTS: DRO significantly reversed MDR in K562/A02 ( P < 0.01). After treatment of DRO 20, 10, and 5 mu mol/L, the chemosensitivity to ADR was increased to 14, 13, and 4 folds, respectively. The reversal activity of DRO was similar to that of verapamil (VRP). After treated with DRO 10 mu mol/L, both MDR1 and GST pi mRNA expression began to decline on the 2nd day, and significantly decreased on the 5th day ( P < 0.01). The changes in P-gp and GST pi protein expression were similar to that of their mRNA expression. Two hours after treatment of DRO 20, 10, and 5 mu mol/L, intracellular ADR concentration in K562/A02 was increased to 2.9, 2.3, and 1.5 folds, respectively. However, DRO did not markedly increase ADR accumulation in K562. CONCLUSION: DRO had strong reversal effect on MDR in K562/A02, which was comparable to that of VRP, but the reversal effect was via different pathways. |
| 语种 | 英语 |
| CSCD记录号 | CSCD:515928 |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/56611]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 推荐引用方式 GB/T 7714 | . Reversal effects of droloxifene on multidrug resistance in adriamycin-resistant K562 cell line[J]. ACTA PHARMACOLOGICA SINICA,2001,22. |
| APA | (2001).Reversal effects of droloxifene on multidrug resistance in adriamycin-resistant K562 cell line.ACTA PHARMACOLOGICA SINICA,22. |
| MLA | "Reversal effects of droloxifene on multidrug resistance in adriamycin-resistant K562 cell line".ACTA PHARMACOLOGICA SINICA 22(2001). |
入库方式： OAI收割
来源：合肥物质科学研究院
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