An efficient protocol of cryo-correlative light and electron microscopy for the study of neuronal synapses
文献类型：期刊论文
| 刊名 | 生物物理学报:英文版
 |
| 出版日期 | 2019 |
| 卷号 | 005 |
| 关键词 | Cryo-correlative light and electron microscopy Cryo-electron tomography Neuronal synapse |
| ISSN号 | 2364-3439 |
| 其他题名 | An efficient protocol of cryo-correlative light and electron microscopy for the study of neuronal synapses |
| 英文摘要 | Cryo-electron tomography is an emerging electron microscopy technique for determining three? dimensional structures of cellular architectures near their native state at nanometer resolution, with a shortcoming of lack of specific labels. Fluorescence light microscopy;on the other hand, specifically visualizes target cellular and molecular components with fluorescent labels, but islim 让 ed to a resolution oftens to hundreds nanometers. Combining the advantages of the two techniques, we have developed a cryo-correlative light and electron microscopy system. Our system consists a custom-designed cryo-chamber that allows for fluorescence imaging of frozen-hydrated samples, and an algorithm to achieve accurate correlation. W让h this system and our optimized protocol, high-quality tomograms of neuronal synapses labelled by specific fluorescent tags in cultured hippocampal neurons are obtained at high efficiency. |
| 语种 | 中文 |
| CSCD记录号 | CSCD:6547467 |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/53035]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 推荐引用方式 GB/T 7714 | . An efficient protocol of cryo-correlative light and electron microscopy for the study of neuronal synapses[J]. 生物物理学报:英文版,2019,005. |
| APA | (2019).An efficient protocol of cryo-correlative light and electron microscopy for the study of neuronal synapses.生物物理学报:英文版,005. |
| MLA | "An efficient protocol of cryo-correlative light and electron microscopy for the study of neuronal synapses".生物物理学报:英文版 005(2019). |
入库方式： OAI收割
来源：合肥物质科学研究院
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