Applications of Locked Nucleic Acid Molecular Beacons in Molecular Recognition and Bioanalysis
文献类型：期刊论文
| 刊名 | PROGRESS IN CHEMISTRY
 |
| 出版日期 | 2015 |
| 卷号 | 27 |
| 关键词 | SINGLE-NUCLEOTIDE POLYMORPHISMS REAL-TIME PCR MODIFIED ANTISENSE OLIGONUCLEOTIDES IN-VIVO MESSENGER-RNA CHEMICAL-MODIFICATION NUCLEOSIDE ANALOGS NONHUMAN-PRIMATES MAMMALIAN-CELLS LNA locked nucleic acid molecular beacons nucleic acids molecular recognition bioanalysis |
| ISSN号 | 1005-281X |
| 其他题名 | Applications of Locked Nucleic Acid Molecular Beacons in Molecular Recognition and Bioanalysis |
| 英文摘要 | Molecular beacons are stem-loop hairpin-structured fluorescence probes with a fluorescent dye at 5' end and a fluorescence quencher at 3' end. When a target complementary sequence is absent, the molecular beacons do not fluoresce, because the formation of the hairpin structure brings the quencher and fluorophore into close proximity, whereby fluorescence is quenched with high efficiency. When a target molecule is present, the hybridization between the target and the loop sequence of the molecular beacon results in the spatial separation of the fluorophore and quencher, which opens the stem-loop structure of molecular beacons to emit fluorescence. Locked nucleic acid is a nucleic acid analogue containing one or more LNA nucleotide monomers with a bicyclic furanose unit locked in an RNA mimicking sugar conformation. It possesses excellent binding affinity to nucleic acid, high biostability and resists to nuclease degradation. Due to the excellent sensitivity and high specificity, the combination of the molecular beacons and locked nucleic acid has aroused wide concern. In this review, we intensively summarize the structure, function, design essentials, current research topics and some important progress. In addition, we also discuss the applications, potential problems and perspective of locked nucleic acid molecular beacons in molecular recognition and bioanalysis. |
| 资助项目 | [National Natural Science Foundation of China] ; [Special Project in Clinic Medicine of Jiangsu Province] ; [Medicine Leading Talents of Jiangsu Province] |
| 语种 | 英语 |
| CSCD记录号 | CSCD:5590828 |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/50554]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 推荐引用方式 GB/T 7714 | . Applications of Locked Nucleic Acid Molecular Beacons in Molecular Recognition and Bioanalysis[J]. PROGRESS IN CHEMISTRY,2015,27. |
| APA | (2015).Applications of Locked Nucleic Acid Molecular Beacons in Molecular Recognition and Bioanalysis.PROGRESS IN CHEMISTRY,27. |
| MLA | "Applications of Locked Nucleic Acid Molecular Beacons in Molecular Recognition and Bioanalysis".PROGRESS IN CHEMISTRY 27(2015). |
入库方式： OAI收割
来源：合肥物质科学研究院
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