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Chinese Academy of Sciences Institutional Repositories Grid
Genotoxic responses to titanium dioxide nanoparticles and fullerene in gpt delta transgenic MEF cells

文献类型:期刊论文

作者Xu,An1,2; Chai,Yunfei3; Nohmi,Takehiko4; Hei,Tom K1,3
刊名Particle and Fibre Toxicology
出版日期2009-01-20
卷号6
ISSN号1743-8977
DOI10.1186/1743-8977-6-3
通讯作者Hei,Tom K(tkh1@columbia.edu)
英文摘要AbstractBackgroundTitanium dioxide (TiO2) nanoparticles and fullerene (C60) are two attractive manufactured nanoparticles with great promise in industrial and medical applications. However, little is known about the genotoxic response of TiO2 nanoparticles and C60 in mammalian cells. In the present study, we determined the mutation fractions induced by either TiO2 nanoparticles or C60 in gpt delta transgenic mouse primary embryo fibroblasts (MEF) and identified peroxynitrite anions (ONOO-) as an essential mediator involved in such process.ResultsBoth TiO2 nanoparticles and C60 dramatically increased the mutation yield, which could be abrogated by concurrent treatment with the endocytosis inhibitor, Nystatin. Under confocal scanning microscopy together with the radical probe dihydrorhodamine 123 (DHR 123), we found that there was a dose-dependent formation of ONOO- in live MEF cells exposed to either TiO2 nanoparticles or C60, and the protective effects of antioxidants were demonstrated by the nitric oxide synthase (NOS) inhibitor, NG-methyl-L-arginine (L-NMMA). Furthermore, suppression of cyclooxygenase-2 (COX-2) activity by using the chemical inhibitor NS-398 significantly reduced mutation frequency of both TiO2 nanoparticles and C60.ConclusionOur results provided novel information that both TiO2 nanoparticles and C60 were taken up by cells and induced kilo-base pair deletion mutations in a transgenic mouse mutation system. The induction of ONOO- may be a critical signaling event for nanoparticle genotoxicity.
语种英语
WOS记录号BMC:10.1186/1743-8977-6-3
出版者BioMed Central
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/42782]  
专题中国科学院合肥物质科学研究院
通讯作者Hei,Tom K
作者单位1.College of Physicians & Surgeons, Columbia University; Center for Radiological Research
2.Chinese Academy of Sciences; Key Laboratory of Ion Beam Bioengineering, Institute of Plasma Physics
3.Mailman School of Public Health, Columbia University; Department of Environmental Health Sciences
4.National Institute of Health Science; Division of Genetics and Mutagenesis
推荐引用方式
GB/T 7714
Xu,An,Chai,Yunfei,Nohmi,Takehiko,et al. Genotoxic responses to titanium dioxide nanoparticles and fullerene in gpt delta transgenic MEF cells[J]. Particle and Fibre Toxicology,2009,6.
APA Xu,An,Chai,Yunfei,Nohmi,Takehiko,&Hei,Tom K.(2009).Genotoxic responses to titanium dioxide nanoparticles and fullerene in gpt delta transgenic MEF cells.Particle and Fibre Toxicology,6.
MLA Xu,An,et al."Genotoxic responses to titanium dioxide nanoparticles and fullerene in gpt delta transgenic MEF cells".Particle and Fibre Toxicology 6(2009).

入库方式: OAI收割

来源:合肥物质科学研究院

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