中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 合肥物质科学研究院
  3. 中国科学院合肥物质科学研究院
Development of marker-free transgenic Jatropha curcas producing curcin-deficient seeds through endosperm-specific RNAi-mediated gene silencing

文献类型：期刊论文

作者Gu,Keyu1; Tian,Dongsheng1; Mao,Huizhu1; Wu,Lifang1,4; Yin,Zhongchao1,2,3
刊名BMC Plant Biology
出版日期2015-10-08
卷号15
关键词Jatropha curcas Curcin RNAi Marker-free transformation Gene silencing Detoxification
ISSN号1471-2229
DOI10.1186/s12870-015-0625-z
通讯作者Yin,Zhongchao(yinzc@tll.org.sg)
英文摘要AbstractBackgroundJatropha curcas L. is a potential biofuel plant and its seed oil is suitable for biodiesel production. Despite this promising application, jatropha seeds contain two major toxic components, namely phorbol esters and curcins. These compounds would reduce commercial value of seed cake and raise safety and environment concerns on jatropha plantation and processing. Curcins are Type I ribosome inactivating proteins. Several curcin genes have been identified in the jatropha genome. Among which, the Curcin 1 (C1) gene is identified to be specifically expressed in endosperm, whereas the Curcin 2A (C2A) is mainly expressed in young leaves.ResultsA marker-free RNAi construct carrying a β-estradiol-regulated Cre/loxP system and a C1 promoter-driven RNAi cassette for C1 gene was made and used to generate marker-free transgenic RNAi plants to specifically silence the C1 gene in the endosperm of J. curcas. Plants of transgenic line L1, derived from T0-1, carry two copies of marker-free RNAi cassette, whereas plants of L35, derived from T0-35, harbored one copy of marker-free RNAi cassette and three copies of closely linked and yet truncated Hpt genes. The C1 protein content in endosperm of L1 and L35 seeds was greatly reduced or undetectable, while the C2A proteins in young leaves of T0-1 and T0-35 plants were unaffected. In addition, the C1 mRNA transcripts were undetectable in the endosperm of T3 seeds of L1 and L35. The results demonstrated that the expression of the C1 gene was specifically down-regulated or silenced by the double-stranded RNA-mediated RNA interference generated from the RNAi cassette.ConclusionThe C1 promoter-driven RNAi cassette for the C1 gene in transgenic plants was functional and heritable. Both C1 transcripts and C1 proteins were greatly down-regulated or silenced in the endosperm of transgenic J. curcas. The marker-free transgenic plants and curcin-deficient seeds developed in this study provided a solution for the toxicity of curcins in jatropha seeds and addressed the safety concerns of the marker genes in transgenic plants on the environments.
语种英语
WOS记录号BMC:10.1186/S12870-015-0625-Z
出版者BioMed Central
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/42780]  
专题中国科学院合肥物质科学研究院
通讯作者Yin,Zhongchao
作者单位1.National University of Singapore; Temasek Life Sciences Laboratory, 1 Research Link
2.National University of Singapore; Department of Biological Sciences
3.Nanyang Technological University; School of Biological Sciences
4.Chinese Academy of Sciences; Present address: Hefei Institutes of Physical Science
推荐引用方式
GB/T 7714		 Gu,Keyu,Tian,Dongsheng,Mao,Huizhu,et al. Development of marker-free transgenic Jatropha curcas producing curcin-deficient seeds through endosperm-specific RNAi-mediated gene silencing[J]. BMC Plant Biology,2015,15.
APA Gu,Keyu,Tian,Dongsheng,Mao,Huizhu,Wu,Lifang,&Yin,Zhongchao.(2015).Development of marker-free transgenic Jatropha curcas producing curcin-deficient seeds through endosperm-specific RNAi-mediated gene silencing.BMC Plant Biology,15.
MLA Gu,Keyu,et al."Development of marker-free transgenic Jatropha curcas producing curcin-deficient seeds through endosperm-specific RNAi-mediated gene silencing".BMC Plant Biology 15(2015).

入库方式： OAI收割

来源：合肥物质科学研究院

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。