Propofol impairs specification of retinal cell types in zebrafish by inhibiting Zisp-mediated Noggin-1 palmitoylation and trafficking
文献类型：期刊论文
| 作者 | Fan,Xiaoqing3; Yang,Haoran1,2 ; Hu,Lizhu1,2; Wang,Delong3; Wang,Ruiting3; Hao,Aijun4; Chen,Xueran1,2
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| 刊名 | Stem Cell Research & Therapy
 |
| 出版日期 | 2021-03-20 |
| 卷号 | 12 |
| 关键词 | Noggin Palmitoylation Propofol Retina Zebrafish Zisp |
| DOI | 10.1186/s13287-021-02204-0 |
| 通讯作者 | Hao,Aijun(aijunhao@sdu.edu.cn) ; Chen,Xueran(xueranchen@cmpt.ac.cn) |
| 英文摘要 | AbstractBackgroundPropofol can have adverse effects on developing neurons, leading to cognitive disorders, but the mechanism of such an effect remains elusive. Here, we aimed to investigate the effect of propofol on neuronal development in zebrafish and to identify the molecular mechanism(s) involved in this pathway.MethodsThe effect of propofol on neuronal development was demonstrated by a series of in vitro and in vivo experiments. mRNA injections, whole-mount in situ hybridization and immunohistochemistry, quantitative real-time polymerase chain reaction, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling, 5-ethynyl-2′-deoxyuridine labeling, co-immunoprecipitation, and acyl–biotin exchange labeling were used to identify the potential mechanisms of propofol-mediated zisp expression and determine its effect on the specification of retinal cell types.ResultsPropofol impaired the specification of retinal cell types, thereby inhibiting neuronal and glial cell formation in retinas, mainly through the inhibition of Zisp expression. Furthermore, Zisp promoted the stabilization and secretion of a soluble form of the membrane-associated protein Noggin-1, a specific palmitoylation substrate.ConclusionsPropofol caused a severe phenotype during neuronal development in zebrafish. Our findings established a direct link between an anesthetic agent and protein palmitoylation in the regulation of neuronal development. This could be used to investigate the mechanisms via which the improper use of propofol might result in neuronal defects. |
| 语种 | 英语 |
| 出版者 | BioMed Central |
| WOS记录号 | BMC:10.1186/S13287-021-02204-0 |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/120093]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Hao,Aijun; Chen,Xueran |
| 作者单位 | 1.Chinese Academy of Sciences; Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health and Medical Technology, Hefei Institutes of Physical Science 2.Chinese Academy of Sciences; Department of Laboratory Medicine, Hefei Cancer Hospital 3.The First Affiliated Hospital of USTC, University of Science and Technology of China (USTC); Department of Anesthesiology, Division of Life Sciences and Medicine 4.Shandong University; Key Laboratory for Experimental Teratology of Ministry of Education, Shandong Key Laboratory of Mental Disorders, Department of Anatomy and Histoembryology, School of Basic Medical Sciences, Cheeloo College of Medicine |
| 推荐引用方式 GB/T 7714 | Fan,Xiaoqing,Yang,Haoran,Hu,Lizhu,et al. Propofol impairs specification of retinal cell types in zebrafish by inhibiting Zisp-mediated Noggin-1 palmitoylation and trafficking[J]. Stem Cell Research & Therapy,2021,12. |
| APA | Fan,Xiaoqing.,Yang,Haoran.,Hu,Lizhu.,Wang,Delong.,Wang,Ruiting.,...&Chen,Xueran.(2021).Propofol impairs specification of retinal cell types in zebrafish by inhibiting Zisp-mediated Noggin-1 palmitoylation and trafficking.Stem Cell Research & Therapy,12. |
| MLA | Fan,Xiaoqing,et al."Propofol impairs specification of retinal cell types in zebrafish by inhibiting Zisp-mediated Noggin-1 palmitoylation and trafficking".Stem Cell Research & Therapy 12(2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
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