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Using ultrasound-targeted microbubble destruction to enhance radiotherapy of glioblastoma

文献类型：期刊论文

作者Peng, Chanjuan1,6; Wu, Yong2,6; Yang, Yang3,6; Li, Ningning4,6; Chen, Xi1,6; Gu, Linhui5,6; Xu, Dong1,6; Yang, Chen1,6
刊名JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
出版日期2021-02-06
关键词Ultrasound Microbubbles Ultrasound therapy Radiation therapy Glioblastoma
ISSN号0171-5216
DOI10.1007/s00432-021-03542-5
通讯作者Xu, Dong(xudong@zjcc.org.cn) ; Yang, Chen(yangchen@zjcc.org.cn)
英文摘要Objective To investigate the efficacy and mechanism of ultrasound-targeted microbubble destruction (UTMD) combined with radiotherapy (XRT) on glioblastoma. Methods The enhanced radiosensitization by UTMD was assessed through colony formation and cell apoptosis in Human glioblastoma cells (U87MG). Subcutaneous transplantation tumors in 24 nude mice implanted with U87MG cells were randomly assigned to 4 different treatment groups (Control, UTMD, XRT, and UTMD + XRT) based on tumor sizes (100-300 mm(3)). Tumor growth was observed for 10 days after treatment, and then histopathology stains (HE, CD34, and gamma H2AX) were applied to the tumor samples. A TUNEL staining experiment was applied to detect the apoptosis rate of mice tumor samples. Meanwhile, tissue proteins were extracted from animal specimens, and the expressions of dsDNA break repair-related proteins from animal specimens were examined by the western blot. Results When the radiotherapy dose was 4 Gy, the colony formation rate of U87MG cells in the UTMD + XRT group was 32 +/- 8%, lower than the XRT group (54 +/- 14%, p < 0.01). The early apoptotic rate of the UTMD + XRT group was 21.1 +/- 3% at 48 h, higher than that of the XRT group (15.2 +/- 4%). The tumor growth curve indicated that the tumor growth was inhibited in the UTMD + XRT group compared with other groups during 10 days of observation. In TUNEL experiment, the apoptotic cells of the UTMD + XRT group were higher than that of the XRT group (p < 0.05). The UTMD + XRT group had the lowest MVD value, but was not significantly different from other groups (p > 0.05). In addition, gamma H2AX increased due to the addition of UTMD to radiotherapy compared to XRT in immunohistochemistry (p < 0.05). Conclusions Our study clearly demonstrated the enhanced destructive effect of UTMD combined with 4 Gy radiotherapy on glioblastoma. This could be partly achieved by the increased ability of DNA damage of tumor cells.
资助项目National Natural Science Foundation of China[81702968] ; Zhejiang Province Medical and Health Science and Technology Project[2017ZD009] ; Zhejiang Province Medical and Health Science and Technology Project[2019KY334]
WOS研究方向Oncology
语种英语
WOS记录号WOS:000615551800004
出版者SPRINGER
资助机构National Natural Science Foundation of China ; Zhejiang Province Medical and Health Science and Technology Project
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/120574]  
专题中国科学院合肥物质科学研究院
通讯作者Xu, Dong; Yang, Chen
作者单位1.Chinese Acad Sci, Dept Ultrasound Med, Canc Hosp, Zhejiang Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China
2.Univ Chinese Acad Sci, Dept Med Engn, Canc Hosp, Zhejiang Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China
3.Univ Chinese Acad Sci, Dept Radiat Oncol, Canc Hosp, Zhejiang Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China
4.Univ Chinese Acad Sci, Canc Hosp, Dept Pathol, Zhejiang Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China
5.Univ Chinese Acad Sci, Dept Core Facil Serv, Canc Hosp, Zhejiang Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China
6.Chinese Acad Sci, Inst Canc & Basic Med IBMC, Hangzhou 310022, Zhejiang, Peoples R China
推荐引用方式
GB/T 7714		 Peng, Chanjuan,Wu, Yong,Yang, Yang,et al. Using ultrasound-targeted microbubble destruction to enhance radiotherapy of glioblastoma[J]. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY,2021.
APA Peng, Chanjuan.,Wu, Yong.,Yang, Yang.,Li, Ningning.,Chen, Xi.,...&Yang, Chen.(2021).Using ultrasound-targeted microbubble destruction to enhance radiotherapy of glioblastoma.JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY.
MLA Peng, Chanjuan,et al."Using ultrasound-targeted microbubble destruction to enhance radiotherapy of glioblastoma".JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY (2021).

入库方式： OAI收割

来源：合肥物质科学研究院

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