Effect of acetate metabolism modulation on 2'-fucosyllactose production in engineered Escherichia coli
文献类型：期刊论文
| 作者 | Liao, Yingxue1,2; Ni, Zhijian1,2; Wu, Jinyong1,3 ; Li, Zhongkui1,2; Ge, Yuanfei1,2; Chen, Xiangsong1,3; Yao, Jianming1,2
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| 刊名 | BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
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| 出版日期 | 2021 |
| 卷号 | 35 |
| 关键词 | 2&apos -Fucosyllactose Escherichia coli acetate culturing medium induction conditions glucose |
| ISSN号 | 1310-2818 |
| DOI | 10.1080/13102818.2021.1885996 |
| 通讯作者 | Chen, Xiangsong(xschen@ipp.ac.cn) ; Yao, Jianming(jmyao@ipp.ac.cn) |
| 英文摘要 | 2'-Fucosyllactose (2'-FL) is one of the most important oligosaccharides in human milk. In this study, a glucose-glycerol mixed carbon source and the deletion of the genes arcA and iclR were attempted for the first time in engineered Escherichia coli to improve 2'-FL production. The highest 2'-FL titre of 5.04 g/L, almost 1.4 times higher than that in the control strain, was obtained in the arcA mutant strain using shake-flask culture. In addition, the arcA mutant showed a significantly reduced accumulation of acetate. However, the deletion of iclR and the double deletion of arcA/iclR provided no benefit in terms of 2'-FL biosynthesis. Overall, these results suggest that deletion of arcA could efficiently increase the 2'-FL yield in E. coli and could lay a foundation for more efficient and larger-scale production. Moreover, in shake-flask culture under optimal conditions, the 2'-FL titer reached a maximum value of 7.14 g/L. |
| 资助项目 | Science and Technology Service Network Initiative of Chinese Academy of Science[KFJ-STS-QYZD-122] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000621623400001 |
| 出版者 | TAYLOR & FRANCIS LTD |
| 资助机构 | Science and Technology Service Network Initiative of Chinese Academy of Science |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/120655]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Chen, Xiangsong; Yao, Jianming |
| 作者单位 | 1.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Plasma Phys, Hefei 230031, Peoples R China 2.USTC, Scinece Isl Branch, Grad Sch, Hefei, Peoples R China 3.Chinese Acad Sci, Inst Plasma Phys, Huainan New Energy Res Ctr, Huainan, Anhui, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liao, Yingxue,Ni, Zhijian,Wu, Jinyong,et al. Effect of acetate metabolism modulation on 2'-fucosyllactose production in engineered Escherichia coli[J]. BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT,2021,35. |
| APA | Liao, Yingxue.,Ni, Zhijian.,Wu, Jinyong.,Li, Zhongkui.,Ge, Yuanfei.,...&Yao, Jianming.(2021).Effect of acetate metabolism modulation on 2'-fucosyllactose production in engineered Escherichia coli.BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT,35. |
| MLA | Liao, Yingxue,et al."Effect of acetate metabolism modulation on 2'-fucosyllactose production in engineered Escherichia coli".BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT 35(2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
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