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Live cell mitochondrial 3-dimensional dynamic ultrastructures under oxidative phosphorylation revealed by a Pyridine-BODIPY probe

文献类型：期刊论文

作者Zhu, Tong5,6; Yang, Guanqing7; Liu, Xiaolu6; Xiang, Pan6; Yang, Zhenghui2,3; Zhang, Sijing7; Chen, Juan7; Wang, Hong7; de Souza, Senio Campos1; Zhang, Zhongping2,3,4
刊名BIOSENSORS & BIOELECTRONICS
出版日期2021-04-15
卷号178
关键词Mitochondrial ultrastructure 3-Dimensional dynamic Oxidative phosphorylation BODIPY-Pyridine derivative STED nanoscopy
ISSN号0956-5663
DOI10.1016/j.bios.2021.113036
通讯作者Tian, Xiaohe(xiaohe.t@ahu.edu.cn)
英文摘要Recent advancements in super-resolution nanoscopy allowed the study of mitochondrial biology at nanoscale and boosted the understanding its correlated cellular processes those were previously poorly understood. Nevertheless, studying mitochondrial ultrastructure remains a challenge due to the lack of probes that could target specific mitochondrial substances (e.g. cristae or mtDNA) and survive under harsh super-resolution optical conditions. Herein, in this work, we have rationally constructed a pyridine-BODIPY (Py-BODIPY) derivative that could target mitochondrial membrane in living cells without interfering its physiological microenvironments. Furthermore, we found Py-BODIPY is a membrane potential independent probe, hence it is not limit to live-cell staining but also showed a strong internalization into pre-fixed and stimulus disrupted sample. Importantly, its cristae specificity and superb photostability allow the observation of mitochondrial dynamic nano-structures with an unprecedented resolution, allow demonstrating how mitochondrial 3D ultrastructure evolved under oxidative phosphorylation condition.
资助项目National Natural Science Foundation of China[51672002] ; National Natural Science Foundation of China[21871003] ; National Natural Science Foundation of China[21701160] ; Doctor Start-up Fund[S020118002/026] ; Hefei National Laboratory for Physical Sciences at the Microscale[KF2019002] ; Anhui University[S020118002/026] ; Anhui University[S020118002/073] ; Natural Science Foundation of Anhui Province of China[1908085MB30] ; Natural Science Foundation of Anhui Province of China[2008085QB52] ; Open Fund For Discipline Construction from Institute of Physical Science and Information Technology, Anhui University
WOS研究方向Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
语种英语
WOS记录号WOS:000621207600005
出版者ELSEVIER ADVANCED TECHNOLOGY
资助机构National Natural Science Foundation of China ; Doctor Start-up Fund ; Hefei National Laboratory for Physical Sciences at the Microscale ; Anhui University ; Natural Science Foundation of Anhui Province of China ; Open Fund For Discipline Construction from Institute of Physical Science and Information Technology, Anhui University
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/120798]  
专题中国科学院合肥物质科学研究院
通讯作者Tian, Xiaohe
作者单位1.UCL, Dept Chem, Gower St, London WC1E 6BT, England
2.Anhui Univ, Inst Phys Sci, Hefei 230601, Peoples R China
3.Anhui Univ, Inst Informat Technol, Hefei 230601, Peoples R China
4.Chinese Acad Sci, CAS Ctr Excellence Nanosci, Inst Intelligent Machines, Hefei, Peoples R China
5.Sichuan Univ, Huaxi MR Res Ctr HMRRC, Dept Radiol, Funct & Mol Imaging Key Lab Sichuan Prov,West Chi, Chengdu 610041, Peoples R China
6.Anhui Univ, Sch Life Sci, Hefei 230601, Peoples R China
7.Anhui Univ, Dept Chem, Key Lab Funct Inorgan Mat Chem Anhui Prov, Hefei 230601, Peoples R China
推荐引用方式
GB/T 7714		 Zhu, Tong,Yang, Guanqing,Liu, Xiaolu,et al. Live cell mitochondrial 3-dimensional dynamic ultrastructures under oxidative phosphorylation revealed by a Pyridine-BODIPY probe[J]. BIOSENSORS & BIOELECTRONICS,2021,178.
APA Zhu, Tong.,Yang, Guanqing.,Liu, Xiaolu.,Xiang, Pan.,Yang, Zhenghui.,...&Tian, Xiaohe.(2021).Live cell mitochondrial 3-dimensional dynamic ultrastructures under oxidative phosphorylation revealed by a Pyridine-BODIPY probe.BIOSENSORS & BIOELECTRONICS,178.
MLA Zhu, Tong,et al."Live cell mitochondrial 3-dimensional dynamic ultrastructures under oxidative phosphorylation revealed by a Pyridine-BODIPY probe".BIOSENSORS & BIOELECTRONICS 178(2021).

入库方式： OAI收割

来源：合肥物质科学研究院

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