Aptamer-functionalized Au nanoparticles array as the effective SERS biosensor for label-free detection of interleukin-6 in serum
文献类型：期刊论文
| 作者 | Muhammad, Muhammad1,2; Shao, Chang-sheng1,2; Huang, Qing1,2
|
| 刊名 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 出版日期 | 2021-05-01 |
| 卷号 | 334 |
| 关键词 | Surface-enhanced Raman spectroscopy (SERS) Au nanoparticles Aptamer Interleukin-6 (IL-6) Biosensor Radiation injury |
| DOI | 10.1016/j.snb.2021.129607 |
| 通讯作者 | Huang, Qing(huangq@ipp.ac.cn) |
| 英文摘要 | A label-free, highly sensitive, and selective biosensor for detection of interleukin-6 (IL-6), which is an important inflammatory and cancer progression cytokine, has been achieved by combining gold nanoparticles (NPs) array surface-enhanced Raman spectroscopy (SERS) substrate and DNA aptamer. The SERS substrate consisted of Au NPs array is functionalized with the aptamer which is composed of the IL-6 recognition sequence and the output signal reporter. Upon recognition of target IL-6 in serum, the aptamer changes its conformation, resulting in the corresponding change of the output Raman intensity ratio (I-660/I-736), with which the quantitative evaluation of IL-6 in 10(-12)-10(-7) M range can be realized. This SERS approach was employed to detect IL-6 in mice serum for inspecting the effect of drug and bacterial infection, and particularly, the radiation injury in the treated animals. This work therefore exemplifies that the aptamer-based SERS biosensor can serve as a promising tool for quick and convenient medical diagnosis applications. |
| 资助项目 | National Natural Science Foundation of China[11635013] ; CSC fellowship from Government of China |
| WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| 语种 | 英语 |
| WOS记录号 | WOS:000632938900007 |
| 出版者 | ELSEVIER SCIENCE SA |
| 资助机构 | National Natural Science Foundation of China ; CSC fellowship from Government of China |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/121298]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Huang, Qing |
| 作者单位 | 1.Univ Sci & Technol China, Grad Sch, Sci Isl Branch, Hefei 230026, Peoples R China 2.Chinese Acad Sci, Inst Intelligent Machines, Hefei Inst Phys Sci, CAS Key Lab High Magnet Field & Ion Beam Phys Bio, Hefei 230031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Muhammad, Muhammad,Shao, Chang-sheng,Huang, Qing. Aptamer-functionalized Au nanoparticles array as the effective SERS biosensor for label-free detection of interleukin-6 in serum[J]. SENSORS AND ACTUATORS B-CHEMICAL,2021,334. |
| APA | Muhammad, Muhammad,Shao, Chang-sheng,&Huang, Qing.(2021).Aptamer-functionalized Au nanoparticles array as the effective SERS biosensor for label-free detection of interleukin-6 in serum.SENSORS AND ACTUATORS B-CHEMICAL,334. |
| MLA | Muhammad, Muhammad,et al."Aptamer-functionalized Au nanoparticles array as the effective SERS biosensor for label-free detection of interleukin-6 in serum".SENSORS AND ACTUATORS B-CHEMICAL 334(2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
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