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NMR Structure and Dynamics Studies of Yeast Respiratory Supercomplex Factor 2

文献类型：期刊论文

作者Zhou, Shu1,2; Pettersson, Pontus2; Huang, Jingjing3; Brzezinski, Peter2; Pomes, Regis3; Maler, Lena2; Adelroth, Pia2
刊名STRUCTURE
出版日期2021-03-04
卷号29
ISSN号0969-2126
DOI10.1016/j.str.2020.08.008
通讯作者Maler, Lena(lena.maler@dbb.su.se) ; Adelroth, Pia(pia.adelroth@dbb.su.se)
英文摘要The Saccharomyces cerevisiae respiratory supercomplex factor 2 (Rcf2) is a 224-residue protein located in the mitochondrial inner membrane where it is involved in the formation of supercomplexes composed of cytochrome bc(1) and cytochrome c oxidase. We previously demonstrated that Rcf2 forms a dimer in dodecylphosphocholine micelles, and here we report the solution NMR structure of this Rcf2 dimer. Each Rcf2 monomer has two soluble alpha helices and five putative transmembrane (TM) alpha helices, including an unexpectedly charged TM helix at the C terminus, which mediates dimer formation. The NOE contacts indicate the presence of inter-monomer salt bridges and hydrogen bonds at the dimer interface, which stabilize the Rcf2 dimer structure. Moreover, NMR chemical shift change mapping upon lipid titrations as well as molecular dynamics analysis reveal possible structural changes upon embedding Rcf2 into a native lipid environment. Our results contribute to the understanding of respiratory supercomplex formation and regulation.
WOS关键词PARTICLE MESH EWALD ; MEMBRANE-PROTEIN ; CHAIN SUPERCOMPLEXES ; SOFTWARE NEWS ; FORCE-FIELD ; SPECTROSCOPY ; ASSIGNMENT ; MODEL ; RCF2 ; C-13
资助项目Knut and Alice Wallenberg Foundation ; Swedish Research Council ; iNEXT[653706] ; Horizon 2020 programme of the European Commission ; Natural Sciences and Engineering Council of Canada (NSERC) ; Hospital for Sick Children
WOS研究方向Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
语种英语
出版者CELL PRESS
WOS记录号WOS:000629154700009
资助机构Knut and Alice Wallenberg Foundation ; Swedish Research Council ; iNEXT ; Horizon 2020 programme of the European Commission ; Natural Sciences and Engineering Council of Canada (NSERC) ; Hospital for Sick Children
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/121310]  
专题中国科学院合肥物质科学研究院
通讯作者Maler, Lena; Adelroth, Pia
作者单位1.Chinese Acad Sci, Hefei Inst Phys Sci, High Magnet Field Lab, Hefei 230031, Peoples R China
2.Stockholm Univ, Dept Biochem & Biophys, S-10691 Stockholm, Sweden
3.Univ Toronto, Hosp Sick Children, Dept Biochem, Mol Med, Toronto, ON M5G 0A4, Canada
推荐引用方式
GB/T 7714		 Zhou, Shu,Pettersson, Pontus,Huang, Jingjing,et al. NMR Structure and Dynamics Studies of Yeast Respiratory Supercomplex Factor 2[J]. STRUCTURE,2021,29.
APA Zhou, Shu.,Pettersson, Pontus.,Huang, Jingjing.,Brzezinski, Peter.,Pomes, Regis.,...&Adelroth, Pia.(2021).NMR Structure and Dynamics Studies of Yeast Respiratory Supercomplex Factor 2.STRUCTURE,29.
MLA Zhou, Shu,et al."NMR Structure and Dynamics Studies of Yeast Respiratory Supercomplex Factor 2".STRUCTURE 29(2021).

入库方式： OAI收割

来源：合肥物质科学研究院

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