Low Dose of Trichostatin A Improves Radiation Resistance by Activating Akt/Nrf2-Dependent Antioxidation Pathway in Cancer Cells
文献类型：期刊论文
| 作者 | Zhang, Fengqiu2,3; Shao, Changsheng1,2; Chen, Zhu1,2; Li, Yalin3; Jing, Xumiao3; Huang, Qing1,2
|
| 刊名 | RADIATION RESEARCH
 |
| 出版日期 | 2021-04-01 |
| 卷号 | 195 |
| ISSN号 | 0033-7587 |
| DOI | 10.1667/RADE-20-00145.1 |
| 通讯作者 | Huang, Qing(huangq@ipp.ac.cn) |
| 英文摘要 | Numerous studies have shown that histone deacetylase inhibitors (HDACis) improve cellular acetylation while also enhancing the radiation sensitivity. In this work, however, we confirmed that low-dose trichostatin A (TSA) as a typical HDACi could reduce rather than increase the radiosensitivity of cancer cells, while the cellular acetylation was also increased with TSA-induced epigenetic modification. The surviving fraction of HeLa/HepG2 cells pretreated with 25 nM TSA for 24 h was higher at 1 Gy/2 Gy of gamma-ray radiation than that of the cells with the same radiation dose but without TSA pretreatment. To understand the underlying mechanism, we investigated the effect of low-dose TSA on HO-1, SOD and CAT induction and activating Akt together with its downstream Nrf2 signaling pathway. Our results indicated that TSA activated HO-1, SOD and CAT expression by increasing the phosphorylation level of Nrf2 in an Akt-dependent manner. In addition, we also observed that the 25-nM-TSA-pretreated group showed a significant increase in the antioxidant capacity in terms of SOD and CAT activities. Therefore, our results suggest that low-dose TSA can activate the Akt/Nrf2 pathway and upregulate expression of HO-1, SOD and CAT to stimulate the cellular defense mechanism. This work demonstrates that low-dose TSA treatment may activate the adaptation mechanism against the oxidative stress induced by ionizing radiation, and application of HDACi treatment should be undertaken with caution to avoid its possible radioresistance in radiotherapy. (C) 2021 by Radiation Research Society |
| 资助项目 | National Natural Science Foundation of China[11635013] ; National Natural Science Foundation of China[11775272] ; National Natural Science Foundation of China[11704343] ; Key Research Project of Universities in Henan province[18A180032] ; Key Scientific and Technological Projects in Henan Province[192102310299] |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics ; Biophysics ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000636622600006 |
| 出版者 | RADIATION RESEARCH SOC |
| 资助机构 | National Natural Science Foundation of China ; Key Research Project of Universities in Henan province ; Key Scientific and Technological Projects in Henan Province |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/121572]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Huang, Qing |
| 作者单位 | 1.Univ Sci & Technol China, Sci Isl Branch, Grad Sch, Hefei 230026, Peoples R China 2.Chinese Acad Sci, Inst Intelligent Machines, CAS Key Lab High Magnet Field & Ion Beam Phys Bio, HFIPS, Hefei 230031, Peoples R China 3.Zhengzhou Univ, Sch Phys, Henan Key Lab Ion Beam Bioengn, Zhengzhou 450052, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Fengqiu,Shao, Changsheng,Chen, Zhu,et al. Low Dose of Trichostatin A Improves Radiation Resistance by Activating Akt/Nrf2-Dependent Antioxidation Pathway in Cancer Cells[J]. RADIATION RESEARCH,2021,195. |
| APA | Zhang, Fengqiu,Shao, Changsheng,Chen, Zhu,Li, Yalin,Jing, Xumiao,&Huang, Qing.(2021).Low Dose of Trichostatin A Improves Radiation Resistance by Activating Akt/Nrf2-Dependent Antioxidation Pathway in Cancer Cells.RADIATION RESEARCH,195. |
| MLA | Zhang, Fengqiu,et al."Low Dose of Trichostatin A Improves Radiation Resistance by Activating Akt/Nrf2-Dependent Antioxidation Pathway in Cancer Cells".RADIATION RESEARCH 195(2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。