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A universal SERS-label immunoassay for pathogen bacteria detection based on Fe3O4@Au-aptamer separation and antibody-protein A orientation recognition

文献类型：期刊论文

作者Zhou, Zihui1,2,3; Xiao, Rui3; Cheng, Siyun3; Wang, Shu4; Shi, Luoluo3; Wang, Chongwen2,3; Qi, Kezong1,2,5; Wang, Shengqi3
刊名ANALYTICA CHIMICA ACTA
出版日期2021-05-22
卷号1160
关键词Surface enhanced Raman scattering Bacteria detection Staphylococcus proteins A Orientation recognition Universal SERS tags
ISSN号0003-2670
DOI10.1016/j.aca.2021.338421
通讯作者Wang, Chongwen(wangchongwen1987@126.com) ; Qi, Kezong(qkz@ahau.edu.cn) ; Wang, Shengqi(sqwang@bmi.ac.cn)
英文摘要Rapid, reliable and sensitive detection methods for pathogenic bacteria are strongly demanded. Herein, we proposed a magnetically assisted surface enhanced Raman scattering (SERS)-label immunoassay for the sensitive detection of bacteria by using a universal approach based on free antibody labelling and staphylococcus proteins A (PA)-SERS tags orientation recognition. The SERS biosensor consists of two functional nanomaterials: aptamer-conjugated Fe3O4@Au magnetic nanoparticles (MNPs) as magnetic SERS platform for pathogen enrichment and PA modified-SERS tags (Au@DTNB@PA) as a universal probe for target bacteria quantitative detection. After target bacteria enriched, free antibody was used to specific marking target bacteria and provided numerous Fc fragment, which can guide the PA-SERS tags orientation-dependent binding. With this strategy, Fe3O4@Au/bacteria/SERS tags sandwich immunocomplexes for most bacteria (expect several species of Staphylococcus) were easy constructed. The limits of detection (LODs) of the proposed assay were found to be 10, 10, and 25 cells/mL for three common pathogens Escherichia coli (E. coli), Listeria monocytogenes (L. mono), and Salmonella typhimurium (S. typhi), respectively, in real food samples. The universal method also exhibits the advantages of rapid, robust , easy to operate, suggesting its great potential for food safety monitoring , infectious diseases diagnosis. (c) 2021 Elsevier B.V. All rights reserved.
资助项目National S&T Major Project for Infectious Diseases Control[2018ZX10712001-010] ; National S&T Major Project for Infectious Diseases Control[2018ZX10101003-001] ; National Natural Science Foundation of China[81830101] ; Natural Science Foundation of Anhui Province[1908085QB85] ; University Synergy Innovation Program of Anhui Province[GXXT-2019-035]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000636834200001
出版者ELSEVIER
资助机构National S&T Major Project for Infectious Diseases Control ; National Natural Science Foundation of China ; Natural Science Foundation of Anhui Province ; University Synergy Innovation Program of Anhui Province
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/121630]  
专题中国科学院合肥物质科学研究院
通讯作者Wang, Chongwen; Qi, Kezong; Wang, Shengqi
作者单位1.Anhui Agr Univ, Coll Anim Sci & Technol, Anhui Prov Engn Lab Anim Food Qual & Biosafety, Hefei 230036, Peoples R China
2.Anhui Agr Univ, Hefei 230036, Peoples R China
3.Beijing Inst Radiat Med, Beijing 100850, Peoples R China
4.Chinese Acad Sci, Hefei Inst Phys Sci, Hefei 230036, Peoples R China
5.Anhui Prov Key Lab Vet Pathobiol & Dis Control, Hefei 230036, Peoples R China
推荐引用方式
GB/T 7714		 Zhou, Zihui,Xiao, Rui,Cheng, Siyun,et al. A universal SERS-label immunoassay for pathogen bacteria detection based on Fe3O4@Au-aptamer separation and antibody-protein A orientation recognition[J]. ANALYTICA CHIMICA ACTA,2021,1160.
APA Zhou, Zihui.,Xiao, Rui.,Cheng, Siyun.,Wang, Shu.,Shi, Luoluo.,...&Wang, Shengqi.(2021).A universal SERS-label immunoassay for pathogen bacteria detection based on Fe3O4@Au-aptamer separation and antibody-protein A orientation recognition.ANALYTICA CHIMICA ACTA,1160.
MLA Zhou, Zihui,et al."A universal SERS-label immunoassay for pathogen bacteria detection based on Fe3O4@Au-aptamer separation and antibody-protein A orientation recognition".ANALYTICA CHIMICA ACTA 1160(2021).

入库方式： OAI收割

来源：合肥物质科学研究院

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