MS2 device: smartphone-facilitated mobile nucleic acid analysis on microfluidic device
文献类型：期刊论文
| 作者 | Wu, Xiaosong1,2 ; Pan, Jingyu2; Zhu, Xinchao1,2; Hong, Chenggang2; Hu, Anzhong2; Zhu, Cancan2; Liu, Yong2; Yang, Ke2; Zhu, Ling2
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| 刊名 | ANALYST
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| 出版日期 | 2021-03-30 |
| ISSN号 | 0003-2654 |
| DOI | 10.1039/d1an00367d |
| 通讯作者 | Yang, Ke(keyang@aiofm.ac.cn) ; Zhu, Ling(zhul@aiofm.ac.cn) |
| 英文摘要 | Mobile sensing based on the integration of microfluidic devices and smartphones, so-called MS2 technology, has enabled many applications over recent years and continues to stimulate growing interest in both research communities and industries. In particular, MS2 technology has been proven to be able to be applied to molecular diagnostic analysis and can be implemented for basic research and clinical testing. However, the currently reported MS2-based nucleic acid analysis system has limited use in practical applications, because it is not integrated with quantitative PCR, multiplex PCR, and isothermal amplification functions, and lacks temperature control, image acquisition and real-time processing units with excellent performance. To provide a more universal and powerful platform, we here developed a novel MS2 device by integrating a thermocycler, a multi fluorescence detection unit, a PCR chip, an isothermal chip, and a smartphone. The MS2 device was approximately 325 mm (L) x 200 mm (W) x 200 mm (H) in volume and only 5 kg in weight, and showed an average power consumption of about 38.4 W. The entire nucleic acid amplification and analysis could be controlled through a self-made smartphone App. The maximum heating and cooling rates were 5 degrees C s(-1) and 4 degrees C s(-1), respectively. The entire PCR could be completed within 65 min. The temperature uniformity was less than 0.1 degrees C. Besides, the temperature stability over time (30 min) was within +/- 0.04 degrees C. Four optical channels were integrated (FAM, HEX, TAMRA, and ROX) on the MS2 device. In particular, the PCR-based detection sensitivity reached 1 copy per mu L, and the amplification efficiency was calculated to be 106.8%. Besides, the MS2 device also was compatible with multiplex PCR and isothermal amplification. In short, the MS2 device showed performance consistent with that of traditional commercial equipment. Thus, the MS2 device provides an easy and integrated experimental platform for molecular diagnostic-related research and potential medical diagnostic applications. |
| 资助项目 | Class C talent fund project of Chinese Academy of Sciences[CXJJ-19-C03] ; Key Research and Development Program of Anhui[1804a09020051] ; Key Research and Development Program of Anhui[1804c04020335] ; Key Research and Development Program of Anhui[201904c03020001] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2018489] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2021442] ; National Natural Science Foundation of China[31800713] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000639954800001 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 资助机构 | Class C talent fund project of Chinese Academy of Sciences ; Key Research and Development Program of Anhui ; Youth Innovation Promotion Association of the Chinese Academy of Sciences ; National Natural Science Foundation of China |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/121720]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Yang, Ke; Zhu, Ling |
| 作者单位 | 1.Univ Sci & Technol China, 96 JinZhai Rd, Hefei 230026, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, Anhui Inst Opt & Fine Mech, Hefei, Anhui, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Xiaosong,Pan, Jingyu,Zhu, Xinchao,et al. MS2 device: smartphone-facilitated mobile nucleic acid analysis on microfluidic device[J]. ANALYST,2021. |
| APA | Wu, Xiaosong.,Pan, Jingyu.,Zhu, Xinchao.,Hong, Chenggang.,Hu, Anzhong.,...&Zhu, Ling.(2021).MS2 device: smartphone-facilitated mobile nucleic acid analysis on microfluidic device.ANALYST. |
| MLA | Wu, Xiaosong,et al."MS2 device: smartphone-facilitated mobile nucleic acid analysis on microfluidic device".ANALYST (2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
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