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A pressure-calibration method of wavelength modulation spectroscopy in sealed microbial growth environment*

文献类型：期刊论文

作者Wang, Kun-Yang1,2,3; Shao, Jie1; Shao, Li-Gang2; Chen, Jia-Jin2; Wang, Gui-Shi2; Liu, Kun2; Gao, Xiao-Ming2,3
刊名CHINESE PHYSICS B
出版日期2021-05-01
卷号30
关键词second harmonic amplitude pressure-calibration microbial growth sealed environment CO2
ISSN号1674-1056
DOI10.1088/1674-1056/abda32
通讯作者Shao, Jie(shaojie@zjnu.cn)
英文摘要A new pressure-calibration method for calibrating the reduction of second harmonic (2f) amplitude caused by pressure broadening effect in sealed microbial growth environment is present. The new method combines with linewidth compensation and modulation depth compensation and makes the 2f amplitude accurately retrieve metabolic CO2 in microbial growth. In order to verify the method, a simulation experiment is developed, in which the increasing CO2 concentration leads to the increasing pressure. Comparing with the relation between the traditional 2f amplitude and gas concentration, there is a monotonous relation between the calibrated 2f amplitude and CO2 concentration, particularly, a linear relation is present when the CO2 concentration is replaced with the CO2 particle number. In terms of microbial measurement, the growth of Escherichia coli is measured, and the culture bottle is sealed during the microbial growth process. The experimental results show that, comparing to the microbial growth retrieved by traditional 2f amplitude, the calibrated 2f amplitude can accurately retrieve microbial growth in sealed environment.
资助项目National Key Research and Development Program of China[2017YFC0209700] ; National Key Research and Development Program of China[2016YFC0303900] ; National Natural Science Foundation of China[41730103]
WOS研究方向Physics
语种英语
WOS记录号WOS:000647651400001
出版者IOP PUBLISHING LTD
资助机构National Key Research and Development Program of China ; National Natural Science Foundation of China
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/121992]  
专题中国科学院合肥物质科学研究院
通讯作者Shao, Jie
作者单位1.Zhejiang Normal Univ, Coll Phys & Elect Informat Engn, Jinhua 321004, Zhejiang, Peoples R China
2.Chinese Acad Sci, Anhui Inst Opt & Fine Mech, Hefei 230031, Peoples R China
3.Univ Sci & Technol China, Hefei 230031, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Kun-Yang,Shao, Jie,Shao, Li-Gang,et al. A pressure-calibration method of wavelength modulation spectroscopy in sealed microbial growth environment*[J]. CHINESE PHYSICS B,2021,30.
APA Wang, Kun-Yang.,Shao, Jie.,Shao, Li-Gang.,Chen, Jia-Jin.,Wang, Gui-Shi.,...&Gao, Xiao-Ming.(2021).A pressure-calibration method of wavelength modulation spectroscopy in sealed microbial growth environment*.CHINESE PHYSICS B,30.
MLA Wang, Kun-Yang,et al."A pressure-calibration method of wavelength modulation spectroscopy in sealed microbial growth environment*".CHINESE PHYSICS B 30(2021).

入库方式： OAI收割

来源：合肥物质科学研究院

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