Rare microbial taxa rather than phoD gene abundance determine hotspots of alkaline phosphomonoesterase activity in the karst rhizosphere soil
文献类型：期刊论文
| 作者 | Liu, Shuang2,3; Zhang, Xinyu2,3 ; Dungait, Jennifer A. J.1; Quine, Timothy A.1; Razavi, Bahar S.4
|
| 刊名 | BIOLOGY AND FERTILITY OF SOILS
 |
| 出版日期 | 2020-11-14 |
| 页码 | 12 |
| 关键词 | phoD gene Phosphate mineralizing Microbial hotspots Rhizosphere Soil zymography |
| ISSN号 | 0178-2762 |
| DOI | 10.1007/s00374-020-01522-4 |
| 通讯作者 | Zhang, Xinyu(zhangxy@igsnrr.ac.cn) |
| 英文摘要 | The spatial distribution of alkaline phosphomonoesterase (ALP) activity in the rhizosphere and bulk soil of three plants (Zea mays, Medicago sativa, and Cyperus rotundus) grown in a karst soil (pH 8.27) was visualized using in situ soil zymography. According to the zymogram images, we identified and precisely collected soil samples from hotspots and non-hotspots of ALP activity, and then analyzed the phoD genes that encoded alkaline phosphomonoesterase and assessed the microbial community. The results showed that (1) the phoD abundance in the plant types varied and was highest in the alfalfa and lowest in the maize; (2) Proteobacteria dominated the phoD-harboring microbes in the rhizosphere and Actinobacteria dominated the phoD-harboring microbes in the bulk soil, and (3) the ALP activity was positively correlated with the relative abundance of the diazotrophic Azotobacter, but negatively correlated with the phoD gene abundance, microbial community richness, and diversity. By coupling zymography and microbial molecular approaches, we identified hotspots of enzymatic and microbial activity in rhizosphere soil and evaluated the relative contributions from potential active microorganisms. We found that the function of specific phoD-harboring microorganisms in these hotspots differed depending on the plant in the soil, which had implications for phosphorus (P) management in P-limited karst soils. The results also suggested that free-living N-2-fixing bacteria (Azotobacter) might promote ALP activity, thereby emphasizing vital linkages between, and coupling of, the soil nutrient cycles. |
| WOS关键词 | ENZYME-ACTIVITY ; PHOSPHORUS FERTILIZATION ; SPATIAL-DISTRIBUTION ; RESOURCE LIMITATION ; PLANT-GROWTH ; MICROORGANISMS ; COMMUNITIES ; MECHANISMS ; ZYMOGRAPHY ; DIVERSITY |
| 资助项目 | Science Centre project of National Natural Science Foundation of China[31988102] ; National Natural Science Foundation of China[41830860] ; National Natural Science Foundation of China[41877091] ; National Natural Science Foundation of China[41571130043] ; National Environmental Research Council of the UK[NE/N007603/1] |
| WOS研究方向 | Agriculture |
| 语种 | 英语 |
| WOS记录号 | WOS:000589505300001 |
| 出版者 | SPRINGER |
| 资助机构 | Science Centre project of National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Environmental Research Council of the UK |
| 源URL | [http://ir.igsnrr.ac.cn/handle/311030/156557]  |
| 专题 | 中国科学院地理科学与资源研究所 |
| 通讯作者 | Zhang, Xinyu |
| 作者单位 | 1.Univ Exeter, Coll Life & Environm Sci, Geog, Rennes Dr, Exeter EX4 4RJ, Devon, England 2.Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Key Lab Ecosyst Network Observat & Modeling, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100190, Peoples R China 4.Univ Kiel, Inst Phytopathol, Dept Soil & Plant Microbiome, Kiel, Germany |
| 推荐引用方式 GB/T 7714 | Liu, Shuang,Zhang, Xinyu,Dungait, Jennifer A. J.,et al. Rare microbial taxa rather than phoD gene abundance determine hotspots of alkaline phosphomonoesterase activity in the karst rhizosphere soil[J]. BIOLOGY AND FERTILITY OF SOILS,2020:12. |
| APA | Liu, Shuang,Zhang, Xinyu,Dungait, Jennifer A. J.,Quine, Timothy A.,&Razavi, Bahar S..(2020).Rare microbial taxa rather than phoD gene abundance determine hotspots of alkaline phosphomonoesterase activity in the karst rhizosphere soil.BIOLOGY AND FERTILITY OF SOILS,12. |
| MLA | Liu, Shuang,et al."Rare microbial taxa rather than phoD gene abundance determine hotspots of alkaline phosphomonoesterase activity in the karst rhizosphere soil".BIOLOGY AND FERTILITY OF SOILS (2020):12. |
入库方式： OAI收割
来源：地理科学与资源研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。