Detection of Exosomes Using Total Internal Reflected Imaging Ellipsometry
文献类型：期刊论文
| 作者 | Liu HY(刘浩宇)1,2; Liu W(刘巍)2 ; Jin G(靳刚)1,2; Jin G(靳刚); Liu W(刘巍)
|
| 刊名 | BIOSENSORS-BASEL
 |
| 出版日期 | 2021-05-01 |
| 卷号 | 11期号:5页码:10 |
| 关键词 | TIRIE exosomes surface protein CD9 CD63 interaction kinetics |
| DOI | 10.3390/bios11050164 |
| 通讯作者 | Jin, Gang(gajin@imech.ac.cn) |
| 英文摘要 | Exosomes are a kind of membrane-bound phospholipid nanovesicle that are secreted extensively in a variety of biological fluids. Accumulating evidence has indicated that exosomes not only communicate with cells, but also perform functional roles in physiology and pathology. In addition, exosomes have also elicited a great deal of excitement due to their potential as disease biomarkers. Therefore, requirements for sensitive methods capable of precisely and specifically determining exosomes were needed. Herein, we not only develop a sensing surface to capture exosomes but also compare two surface proteins on exosomes, which are appropriate for detecting exosome surface markers by total internal reflected imaging ellipsometry (TIRIE). Protein G and antibody were immobilized on a thin layer of golden substrate to form the biosensing surface. The bio-interaction between antibodies and exosomes was recorded by the TIRIE in real time. The distance between exosomes adhered on a surface was 44 nm +/- 0.5 nm. The KD of anti-CD9 and exosome was lower than anti-CD63 and exosome by introducing pseudo-first-order interaction kinetics, which suggested that CD9 is more suitable for exosome surface markers than CD63. The limit of detection (LOD) of TIRIE was 0.4 mu g/mL. In conclusion, we have proposed a surface for the detection of exosomes based on TIRIE, which can make the detection of exosomes convenient and efficient. |
| 分类号 | 二类 |
| WOS关键词 | EXTRACELLULAR VESICLES ; SPR DETECTION ; PROTEINS ; IMMUNOSENSOR |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Instruments & Instrumentation |
| 语种 | 英语 |
| WOS记录号 | WOS:000653707800001 |
| 其他责任者 | Jin, Gang |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/86699]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Sch Engn Sci, 19 Yu Quan Rd, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Mech, NML, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Liu HY,Liu W,Jin G,et al. Detection of Exosomes Using Total Internal Reflected Imaging Ellipsometry[J]. BIOSENSORS-BASEL,2021,11(5):10. |
| APA | 刘浩宇,刘巍,靳刚,Jin G,&Liu W.(2021).Detection of Exosomes Using Total Internal Reflected Imaging Ellipsometry.BIOSENSORS-BASEL,11(5),10. |
| MLA | 刘浩宇,et al."Detection of Exosomes Using Total Internal Reflected Imaging Ellipsometry".BIOSENSORS-BASEL 11.5(2021):10. |
入库方式： OAI收割
来源：力学研究所
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