Multi-color structured illumination microscopy for live cell imaging based on the enhanced image recombination transform algorithm
文献类型:期刊论文
作者 | Zhao, Tianyu3,4,5; Hao, Huiwen2; Wang, Zhaojun5; Liang, Yansheng5; Feng, Kun5; He, Minru3,4; Yun, Xue5; Bianco, Piero R.1; Sun, Yujie2; Yao, Baoli3,4 |
刊名 | Biomedical Optics Express |
出版日期 | 2021-06-01 |
卷号 | 12期号:6页码:3474-3484 |
ISSN号 | 21567085 |
DOI | 10.1364/BOE.423171 |
产权排序 | 1 |
英文摘要 | Structured illumination microscopy (SIM) has attracted considerable interest in superresolution, live-cell imaging because of its low light dose and high imaging speed. Obtaining a high-quality reconstruction image in SIM depends on the precise determination of the parameters of the fringe illumination pattern. The image recombination transform (IRT) algorithm is superior to other algorithms in obtaining the precise initial phase without any approximation, which is promising to provide a considerable solution to address the difficulty of initial phase estimation at low-modulation-depth conditions. However, the IRT algorithm only considers a phase shift of π/2, which limits its applications in general scenarios. In this letter, we present a general form of IRT algorithm suitable for arbitrary phase shifts, providing a powerful tool for parameter estimation in low signal-to-noise cases. To demonstrate the effectiveness of the enhanced IRT algorithm, we constructed a multicolor, structured illumination microscope and studied at super-resolution, the cargo traffic in HRPE cells, and monitored the movement of mitochondrial structures and microtubules in COS-7 cells. The custom SIM system using the enhanced IRT algorithm allows multicolor capability and a low excitation intensity fluorescence imaging less than 1 W/cm2. High-quality super-resolution images are obtained, which demonstrates the utility of this approach in imaging in the life sciences. © 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement |
语种 | 英语 |
出版者 | The Optical Society |
WOS记录号 | WOS:000656912500002 |
源URL | [http://ir.opt.ac.cn/handle/181661/94823] |
专题 | 西安光学精密机械研究所_瞬态光学技术国家重点实验室 |
通讯作者 | Lei, Ming |
作者单位 | 1.Department of Pharmaceutical Sciences, College of Pharmacy, University of Nebraska Medical Center, Omaha; NE; 68198-6025, United States 2.State Key Laboratory of Membrane Biology & Biomedical Pioneer Innovation Center (BIOPIC), School of Life Sciences, Peking University, Beijing; 100871, China; 3.University of Chinese Academy of Sciences, Beijing; 100049, China; 4.State Key Laboratory of Transient Optics and Photonics, Xi’an Institute of Optics and PrecisionMechanics, Chinese Academy of Sciences, Xi’an, Shaanxi; 710119, China; 5.MOE Key Laboratory for Non-equilibrium Synthesis and Modulation of Condensed Matter, School of Physics, Xi’an Jiaotong University, Xi’an; 710049, China; |
推荐引用方式 GB/T 7714 | Zhao, Tianyu,Hao, Huiwen,Wang, Zhaojun,et al. Multi-color structured illumination microscopy for live cell imaging based on the enhanced image recombination transform algorithm[J]. Biomedical Optics Express,2021,12(6):3474-3484. |
APA | Zhao, Tianyu.,Hao, Huiwen.,Wang, Zhaojun.,Liang, Yansheng.,Feng, Kun.,...&Lei, Ming.(2021).Multi-color structured illumination microscopy for live cell imaging based on the enhanced image recombination transform algorithm.Biomedical Optics Express,12(6),3474-3484. |
MLA | Zhao, Tianyu,et al."Multi-color structured illumination microscopy for live cell imaging based on the enhanced image recombination transform algorithm".Biomedical Optics Express 12.6(2021):3474-3484. |
入库方式: OAI收割
来源:西安光学精密机械研究所
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