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Molecular characterization, expression and immune functions of two C-type lectin from Venerupis philippinarum

文献类型：期刊论文

作者Xin, Zifan2; Yu, Daode6; Yang, Bowen2; Chen, Lizhu4; Hayouka, Zvi1; Chen, Xingfu2; Gong, Yitong2; Dai, Haiyu2; Wang, Lei2; Zhao, Yancui2
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2020-12-01
卷号107页码:260-268
关键词Venerupis philippinarum C-type lectin Molecular characterization Immune recognition
ISSN号1050-4648
DOI10.1016/j.fsi.2020.10.006
通讯作者Liu, Xiaoli(lxlshz2006@163.com) ; Yang, Dinglong(dlyang@yic.ac.cn)
英文摘要In the present study, two C-type lectins (designated as VpClec-3 and VpClec-4) were identified and characterized from the manila clam Venerupis philippinarum. Multiple alignment and phylogenetic relationship analysis strongly suggested that VpClec-3 and VpClec-4 belong to the C-type lectin family. In nonstimulated clams, the VpClec-3 transcript was dominantly expressed in the hepatopancreas, while the VpClec-4 transcript was mainly expressed in gill tissues. Both VpClec-3 and VpClec-4 mRNA expression was significantly upregulated following Vibrio anguillarum challenge. Recombinant VpClec-4 (rVpClec-4) was shown to bind lipopolysaccharide (LPS) and glucan in vitro, whereas recombinant VpClec-3 (rVpClec-3) only bound to glucan. In addition, rVpClec-3 and rVpClec-4 displayed broad agglutination activities towards Vibrio harveyi, Vibrio splendidus and V. anguillarum, while no agglutination activities towards Enterobacter cloacae or Aeromonas hydrophila were observed in rVpClec-3. Moreover, hemocyte phagocytosis was significantly enhanced by rVpClec-3 and rVpClec-4. All the results showed that VpClecs function as pattern recognition receptors (PRRs) with distinct recognition spectra and are potentially involved in the innate immune responses of V. philippinarum.
WOS关键词RECOGNITION ; CLONING ; GENE ; HEMOCYTES ; IDENTIFICATION ; DISEASE ; CLAM
资助项目Strategic Priority Research Program of the Chinese Academy of Sciences[XDA23050303] ; National Natural Science Foundation of China[41806196] ; National Natural Science Foundation of China[41506190] ; Natural Science Foundation of Shandong Province[ZR2019BD022] ; Natural Science Foundation of Shandong Province[ZR2017MC045] ; Yantai Science and Technology Development Project[2020MSGY066] ; Marine and Fisheries Science and Technology Innovation Program of Shandong[2017YY04]
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
WOS记录号WOS:000599882200029
资助机构Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province ; Yantai Science and Technology Development Project ; Marine and Fisheries Science and Technology Innovation Program of Shandong
源URL[http://ir.yic.ac.cn/handle/133337/27552]  
专题中国科学院牟平海岸带环境综合试验站
中国科学院烟台海岸带研究所
海岸带生物资源高效利用研究与发展中心
通讯作者Liu, Xiaoli; Yang, Dinglong
作者单位1.Hebrew Univ Jerusalem, Robert H Smith Fac Agr Food & Environm, Inst Biochem Food Sci & Nutr, IL-76100 Rehovot, Israel
2.Ludong Univ, Key Lab Marine Biotechnol Univ Shandong, Sch Life Sci, Yantai 264025, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China
4.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China
5.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China
6.Marine Biol Inst Shandong Prov, Qingdao 266002, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Xin, Zifan,Yu, Daode,Yang, Bowen,et al. Molecular characterization, expression and immune functions of two C-type lectin from Venerupis philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2020,107:260-268.
APA Xin, Zifan.,Yu, Daode.,Yang, Bowen.,Chen, Lizhu.,Hayouka, Zvi.,...&Yang, Dinglong.(2020).Molecular characterization, expression and immune functions of two C-type lectin from Venerupis philippinarum.FISH & SHELLFISH IMMUNOLOGY,107,260-268.
MLA Xin, Zifan,et al."Molecular characterization, expression and immune functions of two C-type lectin from Venerupis philippinarum".FISH & SHELLFISH IMMUNOLOGY 107(2020):260-268.

入库方式： OAI收割

来源：烟台海岸带研究所

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