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Preparation of high-purity R-phycoerythrin and R-phycocyanin fromPyropia yezoensisin membrane chromatography

文献类型：期刊论文

作者Zang, Fan2,3; Qin, Song1,2; Ma, Chengbo2,3; Li, Wenjun1,2; Lin, Jian3
刊名JOURNAL OF APPLIED PHYCOLOGY
出版日期2020-08-03
页码8
关键词Pyropia yezoensis Rhodophyta R-phycoerythrin R-phycocyanin Purification Membrane chromatography
ISSN号0921-8971
DOI10.1007/s10811-020-02208-y
通讯作者Li, Wenjun(wjli@yic.ac.cn) ; Lin, Jian(linjian3384@163.com)
英文摘要R-phycoerythrin and R-phycocyanin are the main light-harvesting pigment-protein complexes inPyropia yezoensis. They have bright colors and a high degree of fluorescence, and are now widely used in biomedicine, food, and cosmetic industries. Therefore, membrane chromatography was used to isolate and purify phycobiliprotein fromP. yezoensis. Algal cells were disrupted by repeated freeze-thaw cycles. Then ammonium sulfate was added into the crude phycobiliprotein extract, so that the saturation of ammonium sulfate in the solution reached 20%, 30%, and 40% in sequence. At 30% and 40% ammonium sulfate saturation, precipitates of R-phycoerythrin and R-phycocyanin mixture in different proportions were obtained. The precipitates were ultra-filtrated to remove the salt, and then, the retentate was taken for membrane chromatographic treatment. In the membrane chromatographic treatment, salt-sensitive PVDF (polyvinyl difluoride) membranes were used for the follow-up experiments. By adjusting the concentration of ammonium sulfate, the membrane system changed the binding strength of various proteins, and then separated different types of phycobiliproteins. The final products of R-phycoerythrin (A(lambda max)/A(280)purity 4.25, yield 45%) and R-phycocyanin (two purity grades: purity 5.8, yield 14.70% and purity 4.18, yield 39.89%) were obtained and then characterized by spectrophotometric and fluorometric measurements and SDS-PAGE. However, the large-scale production of R-phycocyanin and R-phycoerythrin remains a bottleneck as the column chromatography is difficult to be applied for industrial production. This method avoids the problem of easy clogging of column chromatography and can be useful for mass-production of R-phycoerythrin and R-phycocyanin in the future based on the laboratory results.
WOS关键词B-PHYCOERYTHRIN ; MARINE MACROALGAE ; PURIFICATION ; EXTRACTION ; PHYCOBILIPROTEINS
资助项目National Natural Science Foundation of China[41906109] ; Yantai key RD projects[2019XDHZ101] ; Yantai key RD projects[2020MSGY084]
WOS研究方向Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
语种英语
WOS记录号WOS:000555414000002
资助机构National Natural Science Foundation of China ; Yantai key RD projects
源URL[http://ir.yic.ac.cn/handle/133337/28568]  
专题海岸带生物学与生物资源利用重点实验室
烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
中国科学院烟台海岸带研究所
通讯作者Li, Wenjun; Lin, Jian
作者单位1.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
3.Yintai Univ, Coll Life Sci, Yantai 264005, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Zang, Fan,Qin, Song,Ma, Chengbo,et al. Preparation of high-purity R-phycoerythrin and R-phycocyanin fromPyropia yezoensisin membrane chromatography[J]. JOURNAL OF APPLIED PHYCOLOGY,2020:8.
APA Zang, Fan,Qin, Song,Ma, Chengbo,Li, Wenjun,&Lin, Jian.(2020).Preparation of high-purity R-phycoerythrin and R-phycocyanin fromPyropia yezoensisin membrane chromatography.JOURNAL OF APPLIED PHYCOLOGY,8.
MLA Zang, Fan,et al."Preparation of high-purity R-phycoerythrin and R-phycocyanin fromPyropia yezoensisin membrane chromatography".JOURNAL OF APPLIED PHYCOLOGY (2020):8.

入库方式： OAI收割

来源：烟台海岸带研究所

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