Purification and characterization of a novel angiotensin I-converting enzyme-inhibitory peptide derived from Alaska pollack skins
文献类型：期刊论文
| 作者 | Yang, Guilan1,3; Qin, Song1,2 ; Li, Wenjun1,2
|
| 刊名 | JOURNAL OF FOOD SCIENCE
 |
| 出版日期 | 2021-05-30 |
| 页码 | 11 |
| 关键词 | Alaska pollack skins protein hydrolysate angiotensin I-converting enzyme (ACE)-inhibitory peptide gel filtration chromatography molecular docking ultrafiltration |
| ISSN号 | 0022-1147 |
| DOI | 10.1111/1750-3841.15754 |
| 通讯作者 | Li, Wenjun(wjli@yic.ac.cn) |
| 英文摘要 | Hydrolysates containing angiotensin I-converting enzyme (ACE)-inhibitory peptide were prepared from protein of Alaska pollack skins using alcalase and trypsin. The protein hydrolysate was separated by ultrafiltration, Sephadex G-25 gel filtration chromatography and reversed phase high-performance liquid chromatography (HPLC), from which a novel purified peptide was obtained. Both random coil structure and beta-sheet in the purified peptide were revealed in Fourier transform infrared spectrum. The amino sequence of the purified peptide was identified as GPLGVP, VLYPVK, VFLENVLR, and FEEF by HPLC-Q-TOF-MS (HPLC-quadrupole time-of-flight mass spectrometry). The peptide GPLGVP whose molecular weight was 538.31 Da showed the highest ACE inhibitory activity (IC50 = 105.8 mu M). The purified peptide featured a noncompetitive inhibition kinetic mechanism was shown in the Lineweaver-Burk plots and was susceptible to enzymes as indicated in the studies on stability of gastrointestinal proteases. Moreover, the peptide GPLGVP can combine ACE catalytic pocket through hydrogen bonds and other forces with high binding power as disclosed in molecular docking simulation, which provides the inhibitory effect of GPLGVP on ACE. |
| WOS关键词 | PROTEIN HYDROLYSATE ; ACE ; STABILITY ; KINETICS ; MUSCLE ; VENOM |
| 资助项目 | National Key Research and Development Program of China[2018YFD0901102] ; Marine Economy Innovation Development Demonstration Project[YHCX-SW-L-201705] |
| WOS研究方向 | Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000656102700001 |
| 资助机构 | National Key Research and Development Program of China ; Marine Economy Innovation Development Demonstration Project |
| 源URL | [http://ir.yic.ac.cn/handle/133337/29297]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 中国科学院烟台海岸带研究所 |
| 通讯作者 | Li, Wenjun |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 3.Binzhou Med Univ, Sch Pharm, Yantai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Guilan,Qin, Song,Li, Wenjun. Purification and characterization of a novel angiotensin I-converting enzyme-inhibitory peptide derived from Alaska pollack skins[J]. JOURNAL OF FOOD SCIENCE,2021:11. |
| APA | Yang, Guilan,Qin, Song,&Li, Wenjun.(2021).Purification and characterization of a novel angiotensin I-converting enzyme-inhibitory peptide derived from Alaska pollack skins.JOURNAL OF FOOD SCIENCE,11. |
| MLA | Yang, Guilan,et al."Purification and characterization of a novel angiotensin I-converting enzyme-inhibitory peptide derived from Alaska pollack skins".JOURNAL OF FOOD SCIENCE (2021):11. |
入库方式： OAI收割
来源：烟台海岸带研究所
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