Transcriptome analysis reveals hub genes in the hepatopancreas of Exopalaemon carinicauda in response to hypoxia and reoxygenation
文献类型：期刊论文
| 作者 | Shi, Wenjun2,3; Wang, Pan1,2; Hu, Runhao1,2; Wan, Xihe2; Shen, Hui2; Li, Hui2; Wang, Libao2; Qiao, Yi2; Jiang, Ge2; Cheng, Jie2 |
| 刊名 | AQUACULTURE INTERNATIONAL
 |
| 出版日期 | 2021-05-21 |
| 页码 | 27 |
| 关键词 | E carinicauda Hypoxia and reoxygenation RNA-seq WGCNA |
| ISSN号 | 0967-6120 |
| DOI | 10.1007/s10499-021-00717-2 |
| 通讯作者 | Wan, Xihe(wxh1708@126.com) |
| 英文摘要 | Hypoxia is a common stressor in shrimp, but the molecular mechanisms underlying the adaptation of the ridgetail white prawn, Exopalaemon carinicauda, to hypoxia and reoxygenation remain poorly understood. In the present study, ridgetail white prawns were exposed to gradual changes in hypoxia for 6 h via oxygen consumption in a static respiration chamber and then rapidly aerated to allow reoxygenation for 8 h. The dissolved oxygen concentration after 6 h of hypoxia was 1.04 mg/L, and the survival rate of the shrimp was 47%. A transcriptomic analysis of hepatopancreas tissues after 0 (control), 3 (hypoxic starting point), and 6 (hypoxic lethal half point) h of exposure to hypoxia and after 1 and 8 h of reoxygenation was conducted using the Illumina HiSeq(TM) 4000 high-throughput sequencing platform. A total of 93,227 genes were obtained, and 4315 of these genes were identified as differentially expressed genes (DEGs) that respond to hypoxia and reoxygenation. A KEGG pathway enrichment analysis suggested that all identified DEGs were mostly enriched in ribosome biogenesis in eukaryotes, apoptosis, the longevity regulating pathway, the MAPK signaling pathway, and protein processing in the endoplasmic reticulum. In addition, 20,203 genes with RPKM values >= 1 were parsed into 20 modules through a weighted gene coexpression network (WGCNA), and three of these modules were related to hypoxia and reoxygenation. GO and KEGG enrichment analyses of these three modules were then performed, and three hub genes were identified based on their connectivity: RREB1 (Ras-responsive element-binding protein 1-like), UBE1 (ubiquitin-activating enzyme E1), and an unknown gene. This study might help further elucidate the hypoxia tolerance mechanism of E. carinicauda. |
| WOS关键词 | ANTIOXIDANT ENZYME-ACTIVITIES ; SHRIMP LITOPENAEUS-VANNAMEI ; RNA-SEQ DATA ; ENDOPLASMIC-RETICULUM ; ENVIRONMENTAL HYPOXIA ; OXIDATIVE STRESS ; PROTEOMIC ANALYSIS ; DISSOLVED-OXYGEN ; GRASS SHRIMP ; KAPPA-B |
| 资助项目 | Jiangsu Provincial Agricultural Major New Varieties Creation Project[PZCZ201747] ; fifth 226 Project cultivation fund support project of Nantong ; Science and Technology Plan Project of Nantong in 2019[JC2019057] ; Jiangsu Provincial Agricultural Science and Technology Independent Innovation Project in 2018[CX18-2010] |
| WOS研究方向 | Fisheries |
| 语种 | 英语 |
| WOS记录号 | WOS:000652957000001 |
| 资助机构 | Jiangsu Provincial Agricultural Major New Varieties Creation Project ; fifth 226 Project cultivation fund support project of Nantong ; Science and Technology Plan Project of Nantong in 2019 ; Jiangsu Provincial Agricultural Science and Technology Independent Innovation Project in 2018 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/29309]  |
| 专题 | 烟台海岸带研究所_中科院烟台海岸带研究所知识产出 |
| 通讯作者 | Wan, Xihe |
| 作者单位 | 1.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai, Peoples R China 2.Inst Oceanol & Marine Fisheries, 31 Jiaoyu Rd, Nantong 226007, Jiangsu, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Wenjun,Wang, Pan,Hu, Runhao,et al. Transcriptome analysis reveals hub genes in the hepatopancreas of Exopalaemon carinicauda in response to hypoxia and reoxygenation[J]. AQUACULTURE INTERNATIONAL,2021:27. |
| APA | Shi, Wenjun.,Wang, Pan.,Hu, Runhao.,Wan, Xihe.,Shen, Hui.,...&Yang, Zeyu.(2021).Transcriptome analysis reveals hub genes in the hepatopancreas of Exopalaemon carinicauda in response to hypoxia and reoxygenation.AQUACULTURE INTERNATIONAL,27. |
| MLA | Shi, Wenjun,et al."Transcriptome analysis reveals hub genes in the hepatopancreas of Exopalaemon carinicauda in response to hypoxia and reoxygenation".AQUACULTURE INTERNATIONAL (2021):27. |
入库方式： OAI收割
来源：烟台海岸带研究所
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