中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 烟台海岸带研究所
  3. 中国科学院烟台海岸带研究所
  4. 山东省海岸带环境工程技术研究中心
Label-free exonuclease I-assisted signal amplification colorimetric sensor for highly sensitive detection of kanamycin

文献类型：期刊论文

作者Li, Jingwen2; Liu, Yongming2; Lin, Hao2; Chen, Yan3; Liu, Zhenbo2; Zhuang, Xuming2; Tian, Chunyuan2; Fu, Xiuli2; Chen, Lingxin1,4
刊名FOOD CHEMISTRY
出版日期2021-06-15
卷号347页码:6
关键词Kanamycin Exonuclease I Colorimetric Protamine Gold nanoparticles
ISSN号0308-8146
DOI10.1016/j.foodchem.2020.128988
通讯作者Fu, Xiuli(fxl1984.happy@163.com) ; Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要A label-free colorimetric method based on exonuclease I (Exo I)-assisted signal amplification with protamine as a medium was developed for analysis of kanamycin. In this study, a double-stranded DNA (dsDNA) probe was tailored by manipulating an aptamer and its complementary DNA (cDNA) ensuring detection of target with high selectivity and excellent sensitivity. Herein, protamine could not only combine with negatively charged gold nanoparticles but also interaction with polyanion DNA. Upon addition of target kanamycin, the target-aptamer complex was formed and the cDNA was released. Thus, both aptamer and cDNA could be digested by Exo I, and the captured kanamycin was liberated for triggering target recycling and signal amplification. Under optimized conditions, the proposed colorimetric method realized a low detection limit of 2.8 x 10(-14) M along with a wide linear range plus excellent selectivity. Our strategy exhibited enormous potentials for fabricate various kinds of biosensors based on target-induced aptamer configuration changes.
资助项目National Natural Science Foundation of China[21705139] ; National Natural Science Foundation of China[61801274] ; National Natural Science Foundation of China[21778047] ; Natural Science Foundation of Shandong Province in China[ZR2017BB026] ; Natural Science Foundation of Shandong Province in China[ZR2016CB25] ; Qingchuang Science and Technology Program of Shandong Province in China[2019KJF029] ; Taishan Scholar Project Special Funding[ts20190962]
WOS研究方向Chemistry ; Food Science & Technology ; Nutrition & Dietetics
语种英语
WOS记录号WOS:000617924800006
资助机构National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province in China ; Qingchuang Science and Technology Program of Shandong Province in China ; Taishan Scholar Project Special Funding
源URL[http://ir.yic.ac.cn/handle/133337/27402]  
专题烟台海岸带研究所_山东省海岸带环境工程技术研究中心
烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者Fu, Xiuli; Chen, Lingxin
作者单位1.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China
2.Yantai Univ, Sch Chem & Chem Engn, Yantai 264005, Peoples R China
3.Shandong Agr & Engn Univ, Coll Resources & Environm Engn, Jinan 250100, Peoples R China
4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714		 Li, Jingwen,Liu, Yongming,Lin, Hao,et al. Label-free exonuclease I-assisted signal amplification colorimetric sensor for highly sensitive detection of kanamycin[J]. FOOD CHEMISTRY,2021,347:6.
APA Li, Jingwen.,Liu, Yongming.,Lin, Hao.,Chen, Yan.,Liu, Zhenbo.,...&Chen, Lingxin.(2021).Label-free exonuclease I-assisted signal amplification colorimetric sensor for highly sensitive detection of kanamycin.FOOD CHEMISTRY,347,6.
MLA Li, Jingwen,et al."Label-free exonuclease I-assisted signal amplification colorimetric sensor for highly sensitive detection of kanamycin".FOOD CHEMISTRY 347(2021):6.

入库方式： OAI收割

来源：烟台海岸带研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。