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Revisiting the cellular toxicity of benzo[a]pyrene from the view of nanoclusters: size- and nanoplastic adsorption-dependent bioavailability

文献类型：期刊论文

作者Ji, Yunxia1,5; Wang, Yunqing1,2; Shen, Dazhong5; Kang, Qi5; Ma, Jiping3; Chen, Lingxin1,2,4
刊名NANOSCALE
出版日期2021-01-14
卷号13期号:2页码:1016-1028
ISSN号2040-3364
DOI10.1039/d0nr06747d
通讯作者Kang, Qi(kangqi@sdnu.edu.cn) ; Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要Benzo[a]pyrene (Bap) is one of the main organic pollutants in the atmospheric haze that is rich in fine water drops and particulate matters. The understanding of the Bap's form in water is of great importance to unveil its real biological effects toward the respiratory system. To date, various reports have documented its toxicological effects in the molecular form. Herein, we found that Bap existed as self-aggregated nanoclusters of tunable sizes rather than as dissolved molecules in water and different sized nanoclusters illustrated varied cytotoxicity. These findings indicated that the size, which has been ignored in previous studies, is also a dominant parameter similar to the molecular concentration for determining Bap's cytotoxicity. Polystyrene (PS) nanoparticles, as a model for nanoplastics, could adsorb Bap nanoclusters and serve as carriers that enter the cells. The combination effect interestingly altered the cytotoxicity distinction of Bap of different sizes. The intracellular fate of the nanoparticles and subcellular organelle damages were studied to unveil the mechanisms of cytotoxic distinction. Small Bap nanoclusters entered cells faster than their large counterparts. The Bap of the PS@Bap complex was stably adsorbed on PS at the early stages of endocytosis until it was detached during the lysosomal transport and maturation process. The dissociated Bap may bypass the lysosome pathway and be released into the cytosol with a nanocluster structure or relocate into the endoplasmic reticulum. On the other hand, the detached PS preferred to bind to the mitochondria or be excreted out of the cell via the lysosomal pathway. Moreover, the PS@Bap complex resulted in a significant loss of the mitochondrial membrane potential and induced apoptosis through the mitochondria-involved apoptosis pathway. This study provides a new perspective towards the toxicological mechanism of insoluble hydrophobic organic compounds and reveals the environmental significance of nanoplastics for regulating the biological effects of conventional pollutants.
WOS关键词MULTIPHOTON SPECTRAL-ANALYSIS ; GRAPHENE OXIDE ; MICROPLASTICS ; NANOPARTICLES ; SURFACE ; METABOLISM ; CLUSTERS ; CELLS ; PATHWAYS ; DYNAMICS
资助项目National Nature Science Foundation of China[42076199] ; National Nature Science Foundation of China[21976209] ; National Nature Science Foundation of China[21874083] ; National Nature Science Foundation of China[21575080] ; National Nature Science Foundation of China[21976099] ; National Nature Science Foundation of China[81573393] ; program of Youth Innovation Promotion Association, CAS[2017256] ; Instrument Developing Project of the Chinese Academy of Sciences[YZ201662] ; Taishan Scholar Project Special Funding[ts20190962]
WOS研究方向Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
语种英语
WOS记录号WOS:000610368100044
资助机构National Nature Science Foundation of China ; program of Youth Innovation Promotion Association, CAS ; Instrument Developing Project of the Chinese Academy of Sciences ; Taishan Scholar Project Special Funding
源URL[http://ir.yic.ac.cn/handle/133337/27471]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
烟台海岸带研究所_山东省海岸带环境工程技术研究中心
通讯作者Kang, Qi; Chen, Lingxin
作者单位1.Chinese Acad Sci, Shandong Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
3.Qingdao Univ Technol, Sch Environm & Municipal Engn, Qingdao 266033, Peoples R China
4.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
5.Shandong Normal Univ, Key Lab Mol & Nano Probes, Minist Educ, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Peoples R China
推荐引用方式
GB/T 7714		 Ji, Yunxia,Wang, Yunqing,Shen, Dazhong,et al. Revisiting the cellular toxicity of benzo[a]pyrene from the view of nanoclusters: size- and nanoplastic adsorption-dependent bioavailability[J]. NANOSCALE,2021,13(2):1016-1028.
APA Ji, Yunxia,Wang, Yunqing,Shen, Dazhong,Kang, Qi,Ma, Jiping,&Chen, Lingxin.(2021).Revisiting the cellular toxicity of benzo[a]pyrene from the view of nanoclusters: size- and nanoplastic adsorption-dependent bioavailability.NANOSCALE,13(2),1016-1028.
MLA Ji, Yunxia,et al."Revisiting the cellular toxicity of benzo[a]pyrene from the view of nanoclusters: size- and nanoplastic adsorption-dependent bioavailability".NANOSCALE 13.2(2021):1016-1028.

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来源：烟台海岸带研究所

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